X-Linked gene activity in metamales (XY; 3A) of Drosophila

John C. Lucchesi, John M. Belote, Gustavo Maroni

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

In order to determine the level of X-linked gene activity in metamales (XY; 3A) of Drosophila melanogaster, RNA synthesis was monitored as a function of 3H-uridine incorporation in nuclei of isolated larval salivary glands by standard autoradiographic means. These data show that the rate of RNA synthesis by the single X chromosome in metamales is approximately 90% of the rate achieved by the three X chromosomes of triploid females (3X; 3A). As an independent measure of chromosome activity, two X-linked enzymes and two autosomal enzymes were monitored in crude extracts by spectrophotometric assay procedures. The results reveal that X-linked enzyme activity levels in metamales constitute 80% of the levels exhibited by control triploid females or intersexes (XXY; 3A). We conclude that metamales exhibit substantial compensation beyond that which occurs in regular, diploid males. This finding establishes a new level of transcriptional activity possible for the X chromosome of Drosophila melanogaster.

Original languageEnglish (US)
Pages (from-to)1-7
Number of pages7
JournalChromosoma
Volume65
Issue number1
DOIs
StatePublished - Mar 1977
Externally publishedYes

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X-Linked Genes
X Chromosome
Drosophila
Triploidy
Drosophila melanogaster
Enzymes
RNA
Uridine
Salivary Glands
Complex Mixtures
Diploidy
Chromosomes

ASJC Scopus subject areas

  • Genetics

Cite this

Lucchesi, J. C., Belote, J. M., & Maroni, G. (1977). X-Linked gene activity in metamales (XY; 3A) of Drosophila. Chromosoma, 65(1), 1-7. https://doi.org/10.1007/BF00293126

X-Linked gene activity in metamales (XY; 3A) of Drosophila. / Lucchesi, John C.; Belote, John M.; Maroni, Gustavo.

In: Chromosoma, Vol. 65, No. 1, 03.1977, p. 1-7.

Research output: Contribution to journalArticle

Lucchesi, JC, Belote, JM & Maroni, G 1977, 'X-Linked gene activity in metamales (XY; 3A) of Drosophila', Chromosoma, vol. 65, no. 1, pp. 1-7. https://doi.org/10.1007/BF00293126
Lucchesi, John C. ; Belote, John M. ; Maroni, Gustavo. / X-Linked gene activity in metamales (XY; 3A) of Drosophila. In: Chromosoma. 1977 ; Vol. 65, No. 1. pp. 1-7.
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