UV-Assisted direct-write assembly of scaffold-templated nanoclay composites via biotin-streptavidin interactions

Hamid Dalir, Roohollah Dermanaki Farahani, Martin Lévesque, Daniel Therriault

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Scopus citations

Abstract

Three-dimensional epoxy scaffolds with abundant active epoxy groups on surfaces were fabricated through UV-Assisted direct-write manufacturing process. The prepared scaffolds composed of cylindrical filaments (diameter ∼100 μm) were aminated by reacting the epoxy groups with 1,3-diamino-propane. The resulting aminated scaffolds were subsequently biotinylated and then successfully applied to immobilize biotinylated nanoclay conjugates via a specific, strong and rapid binding of biotin and streptavidin. In another approach, the same amount of nanoclays was properly dispersed in epoxy by three-roll mill machine inducing high shear mixing. The nanoclay-epoxy filaments were then deposited by a computerized-control robot in a 3D micro structure scaffold form. Tensile mechanical tests were performed with a dynamic mechanical analysis (DMA) using a film tension clamp on three microstructures: nanoclay-epoxy scaffolds, aminated-biotinylated nanoclays coated on unloaded epoxy scaffolds and finally unloaded epoxy scaffolds (used as a reference). DMA tensile measurements indicated a slight improvement in modulus (by ∼5%), but significant increase in strength (by ∼24%), fracture strain (by ∼21%) and fracture energy (by ∼38%) by introducing biotin-streptavidin strong bonds among epoxy scaffolds and nanoclays in comparison with those of mixed nanoclay-epoxy scaffolds. These mechanical improvements are attributed to the strong biotin and streptavidin bonds between the epoxy scaffolds and nanoclays.

Original languageEnglish (US)
Title of host publicationASME 2010 International Mechanical Engineering Congress and Exposition, IMECE 2010
Pages899-904
Number of pages6
DOIs
StatePublished - Dec 1 2010
EventASME 2010 International Mechanical Engineering Congress and Exposition, IMECE 2010 - Vancouver, BC, Canada
Duration: Nov 12 2010Nov 18 2010

Publication series

NameASME International Mechanical Engineering Congress and Exposition, Proceedings (IMECE)
Volume10

Other

OtherASME 2010 International Mechanical Engineering Congress and Exposition, IMECE 2010
CountryCanada
CityVancouver, BC
Period11/12/1011/18/10

ASJC Scopus subject areas

  • Mechanical Engineering

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    Dalir, H., Farahani, R. D., Lévesque, M., & Therriault, D. (2010). UV-Assisted direct-write assembly of scaffold-templated nanoclay composites via biotin-streptavidin interactions. In ASME 2010 International Mechanical Engineering Congress and Exposition, IMECE 2010 (pp. 899-904). (ASME International Mechanical Engineering Congress and Exposition, Proceedings (IMECE); Vol. 10). https://doi.org/10.1115/IMECE2010-39103