TY - JOUR
T1 - Use of recombinant human soluble TNF receptor in anorectic tumor-bearing rats
AU - Torelli, Giovanni F.
AU - Meguid, Michael M.
AU - Moldawer, Lyle L.
AU - Edwards, Carl K.
AU - Kim, Hyune Ju
AU - Carter, Janna L.
AU - Laviano, Alessandro
AU - Fanelli, Filippo Rossi
PY - 1999/9
Y1 - 1999/9
N2 - With progression of tumor growth, rats demonstrate anorexia and reduced food intake, a function of meal number and meal size. Tumor necrosis factor- α (TNF-α), a recognized anorectic agent, reacts with two different receptors (type I: 55 kDa; type II: 75 kDa). We used a dimeric, pegylated 55- kDa TNF receptor construct to test its effects on food intake, meal number, and meal size, which were continuously measured with a rat eater meter in 16 Fischer 344 male rats injected with 106 viable methylcholanthrene cells. When anorexia developed, rats received a subcutaneous injection of either 0.25 mg/kg body wt of soluble TNF receptor construct (study) or vehicle (tumor-bearing control). Before TNF inhibitor injection, no differences were observed in food intake, meal number, or meal size between the two groups. After the TNF inhibitor injection, study vs. control rats significantly improved food intake as a result of an increase in meal number and meal size. Rats also showed a significant improvement in body weight. These data suggest that TNF-α, in addition to other cytokines, contributes to the anorexia of tumor growth, probably mediated via the hypothalamus.
AB - With progression of tumor growth, rats demonstrate anorexia and reduced food intake, a function of meal number and meal size. Tumor necrosis factor- α (TNF-α), a recognized anorectic agent, reacts with two different receptors (type I: 55 kDa; type II: 75 kDa). We used a dimeric, pegylated 55- kDa TNF receptor construct to test its effects on food intake, meal number, and meal size, which were continuously measured with a rat eater meter in 16 Fischer 344 male rats injected with 106 viable methylcholanthrene cells. When anorexia developed, rats received a subcutaneous injection of either 0.25 mg/kg body wt of soluble TNF receptor construct (study) or vehicle (tumor-bearing control). Before TNF inhibitor injection, no differences were observed in food intake, meal number, or meal size between the two groups. After the TNF inhibitor injection, study vs. control rats significantly improved food intake as a result of an increase in meal number and meal size. Rats also showed a significant improvement in body weight. These data suggest that TNF-α, in addition to other cytokines, contributes to the anorexia of tumor growth, probably mediated via the hypothalamus.
KW - Cancer anorexia
KW - Feeding behavior
KW - Food intake regulation
KW - Tumor necrosis factor-α
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U2 - 10.1152/ajpregu.1999.277.3.r850
DO - 10.1152/ajpregu.1999.277.3.r850
M3 - Article
C2 - 10484503
AN - SCOPUS:0032867672
SN - 0363-6119
VL - 277
SP - R850-R855
JO - American Journal of Physiology - Regulatory Integrative and Comparative Physiology
JF - American Journal of Physiology - Regulatory Integrative and Comparative Physiology
IS - 3 46-3
ER -