@article{fe04f8e77f0648ac931e4ccfeecf47f8,
title = "Unique Role of Vimentin Networks in Compression Stiffening of Cells and Protection of Nuclei from Compressive Stress",
abstract = "In this work, we investigate whether stiffening in compression is a feature of single cells and whether the intracellular polymer networks that comprise the cytoskeleton (all of which stiffen with increasing shear strain) stiffen or soften when subjected to compressive strains. We find that individual cells, such as fibroblasts, stiffen at physiologically relevant compressive strains, but genetic ablation of vimentin diminishes this effect. Further, we show that unlike networks of purified F-actin or microtubules, which soften in compression, vimentin intermediate filament networks stiffen in both compression and extension, and we present a theoretical model to explain this response based on the flexibility of vimentin filaments and their surface charge, which resists volume changes of the network under compression. These results provide a new framework by which to understand the mechanical responses of cells and point to a central role of intermediate filaments in response to compression.",
keywords = "AFM, compression-stiffening, compressive stress, intermediate filaments, vimentin",
author = "Katarzyna Pogoda and Fitzroy Byfield and Piotr Deptu{\l}a and Mateusz Cie{\'s}luk and {\L}ukasz Suprewicz and Karol Sk{\l}odowski and Shivers, {Jordan L.} and {Van Oosten}, Anne and Katrina Cruz and Ekaterina Tarasovetc and Grishchuk, {Ekaterina L.} and MacKintosh, {Fred C.} and Robert Bucki and Patteson, {Alison E.} and Janmey, {Paul A.}",
note = "Funding Information: This work was partially supported by National Science Center of Poland: UMO-2020/01/0/NZ6/00082 (to R.B.) and UMO-2017/26/D/ST4/00997 (to K.P.). Part of the study was conducted with equipment purchased by the Medical University of Bialystok as part of the RPOWP 2007-2013 funding, Priority I, Axis 1.1, contract No. UDA- RPPD.01.01.00-20-001/15-00 dated 26.06.2015. J.L.S. and F.C.M. were supported in part by the National Science Foundation Division of Materials Research (Grant DMR-1826623) and the National Science Foundation Center for Theoretical Biological Physics (Grant PHY2019745). P.A.J. and E.L.G. were supported by NSF-16 DMR-1720530. P.A.J. and F.J.B. were supported by NIH R35 GM136259. A.P. was supported by NIH R35 GM142963. The authors are also grateful to Anatoly Zaytsev for technical assistance during protein purification. Publisher Copyright: {\textcopyright} 2022 The Authors. Published by American Chemical Society.",
year = "2022",
month = jun,
day = "22",
doi = "10.1021/acs.nanolett.2c00736",
language = "English (US)",
volume = "22",
pages = "4725--4732",
journal = "Nano Letters",
issn = "1530-6984",
publisher = "American Chemical Society",
number = "12",
}