Ultrahigh Resolution 3D Model of Murine Heart from Micro-CT and Serial Confocal Laser Scanning Microscopy Images

A. H. Poddar, A. Krol, J. Beaumont, R. L. Price, M. A. Slamani, J. Fawcett, A. Subramanian, I. L. Coman, E. D. Lipson, D. H. Feiglin

Research output: Chapter in Book/Entry/PoemConference contribution

9 Scopus citations

Abstract

This study involves the reconstruction of a distortion-free ultrahigh-resolution 3D model of a whole murine heart. This is achieved by multimodal registration of serial images generated by confocal laser scanning microscopy (CLSM) with the aid of a micro-CT 3D image as a template. High-resolution information from CLSM is utilized for study of fine soft tissue structures in 3D, including fiber orientation and gap junctions. CLSM requires physical sectioning of the sample resulting in missing tissue and in various degrees of tissue distortion depending on thickness. The micro-CT data are distortion free and provide complete information on whole-object interfaces both external and internal. However, they do not provide information on the soft-tissue fine structure. In this project, we used a micro-CT image as template to spatially co-register all the individual CLSM images and to correct the resulting volume for distortion.

Original languageEnglish (US)
Title of host publication2005 IEEE Nuclear Science Symposium Conference Record -Nuclear Science Symposium and Medical Imaging Conference
Pages2615-2617
Number of pages3
DOIs
StatePublished - 2005
EventNuclear Science Symposium Conference Record, 2005 IEEE - , Puerto Rico
Duration: Oct 23 2005Oct 29 2005

Publication series

NameIEEE Nuclear Science Symposium Conference Record
Volume5
ISSN (Print)1095-7863

Other

OtherNuclear Science Symposium Conference Record, 2005 IEEE
Country/TerritoryPuerto Rico
Period10/23/0510/29/05

Keywords

  • 3D large volume high-resolution reconstruction
  • Confocal laser scanning microscopy
  • Micro-CT template for distortion correction

ASJC Scopus subject areas

  • Radiation
  • Nuclear and High Energy Physics
  • Radiology Nuclear Medicine and imaging

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