Ultrafast laser-based micro-CT system for small animal imaging

Andrzej Krol, J. C. Kieffer, J. Nees, L. Chen, R. Toth, B. Hou, R. E. Kincaid, I. L. Coman, E. D. Lipson, G. Mourou

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Scopus citations


We investigated ultrafast laser-based x-ray (ULX) source as an attractive alternative to a microfocal x-ray tube used in micro-CT systems. The laser pulse duration was in the 30 fs-200 fs range, the repetition rate in the 10 Hz - 1 kHz range. A number of solid targets including Ge, Mo, Rh, Ag, Sn, Ba, La, Nd with matching filters was used. We optimized conditions for x-rays generation and measured: x-ray spectra, conversion efficiency (from laser light to x-rays), x-ray fluence, effective x-ray focal spot size and spatial resolution, contrast resolution and radiation dose. Good quality projection images of small animals in single- and dual-energy mode were obtained. ULX generates narrow x-ray spectra that consist mainly of characteristic lines that can be easily tailored (by changing laser beam target) to the imaging task, (e.g. to maximize contrast while minimizing radiation dose). X-ray fluence can exceed fluence produced by conventional microfocal tube with 10 μm focal-spot hence allowing for faster scans with very high spatial resolution. Changing the laser target, and thus matching the characteristic emission lines with the investigated animal's thickness and composition, can be done quickly and easily. Using narrow emission lines for imaging, instead of broad bremsstrahlung, offers superior dose utilization and limits beam-hardening effects. Employing two narrow emission lines - above and below the absorption edge of a contrast agent - in quick succession allows dual-energy-subtraction micro-CT for imaging with a contrast medium. Dual-energy-subtraction is not practical with a microfocal tube. Compact, robust, ultrafast lasers are commercially available, and their characteristics are rapidly improving. We plan to construct a prototype in vivo ultrafast laser-based micro-CT system.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsM.J. Yaffe, M.J. Flynn
Number of pages7
StatePublished - 2004
EventMedical Imaging 2004: Physics of Medical Imaging - San Diego, CA, United States
Duration: Feb 15 2004Feb 17 2004


OtherMedical Imaging 2004: Physics of Medical Imaging
CountryUnited States
CitySan Diego, CA



  • Dual energy CT
  • Micro-CT
  • Ultrafast laser-based x-ray source

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Krol, A., Kieffer, J. C., Nees, J., Chen, L., Toth, R., Hou, B., Kincaid, R. E., Coman, I. L., Lipson, E. D., & Mourou, G. (2004). Ultrafast laser-based micro-CT system for small animal imaging. In M. J. Yaffe, & M. J. Flynn (Eds.), Proceedings of SPIE - The International Society for Optical Engineering (1 ed., Vol. 5368, pp. 265-271) https://doi.org/10.1117/12.536190