Transfer of the gene for neomycin resistance into goldfish, Carassius auratus

S. J. Yoon; E. M. Hallerman; M. L. Gross; Z. Liu; J. F. Schneider; A. J. Faras; P. B. Hackett; A. R. Kapuscinski; K. S. Guise

doi:10.1016/0044-8486(90)90004-7

Transfer of the gene for neomycin resistance into goldfish, Carassius auratus

S. J. Yoon, E. M. Hallerman, M. L. Gross, Z. Liu, J. F. Schneider, A. J. Faras, P. B. Hackett, A. R. Kapuscinski, K. S. Guise

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

A recombinant DNA construct containing the neo gene controlled by a promoter from the Rous sarcoma virus (RSV) was microinjected into newly fertilized, dechorionated goldfish (Carassius auratus) eggs. The neo gene confers resistance to the neomycin analog drug G-418. Results of Southern blot analyses were consistent with incorporation of single or multiple copies of the gene into the genomic DNA of one examined fish. Evidence of neo mRNA in RNA dot-blot analysis indicated that the RSV promoter had initiated transcription within a piscine genome. The utility of the neo gene as a selectable marker for transgenic fish was evaluated by G-418 selection on newly hatched and juvenile fish but proved inconclusive. Reasons for the discrepancy between neo expression and G-418 selection results are discussed.

Original language	English (US)
Pages (from-to)	21-33
Number of pages	13
Journal	Aquaculture
Volume	85
Issue number	1-4
DOIs	https://doi.org/10.1016/0044-8486(90)90004-7
State	Published - Mar 1990
Externally published	Yes

ASJC Scopus subject areas

Aquatic Science

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10.1016/0044-8486(90)90004-7

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title = "Transfer of the gene for neomycin resistance into goldfish, Carassius auratus",

abstract = "A recombinant DNA construct containing the neo gene controlled by a promoter from the Rous sarcoma virus (RSV) was microinjected into newly fertilized, dechorionated goldfish (Carassius auratus) eggs. The neo gene confers resistance to the neomycin analog drug G-418. Results of Southern blot analyses were consistent with incorporation of single or multiple copies of the gene into the genomic DNA of one examined fish. Evidence of neo mRNA in RNA dot-blot analysis indicated that the RSV promoter had initiated transcription within a piscine genome. The utility of the neo gene as a selectable marker for transgenic fish was evaluated by G-418 selection on newly hatched and juvenile fish but proved inconclusive. Reasons for the discrepancy between neo expression and G-418 selection results are discussed.",

author = "Yoon, {S. J.} and Hallerman, {E. M.} and Gross, {M. L.} and Z. Liu and Schneider, {J. F.} and Faras, {A. J.} and Hackett, {P. B.} and Kapuscinski, {A. R.} and Guise, {K. S.}",

year = "1990",

month = mar,

doi = "10.1016/0044-8486(90)90004-7",

language = "English (US)",

volume = "85",

pages = "21--33",

journal = "Aquaculture",

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publisher = "Elsevier",

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T1 - Transfer of the gene for neomycin resistance into goldfish, Carassius auratus

AU - Yoon, S. J.

AU - Hallerman, E. M.

AU - Gross, M. L.

AU - Liu, Z.

AU - Schneider, J. F.

AU - Faras, A. J.

AU - Hackett, P. B.

AU - Kapuscinski, A. R.

AU - Guise, K. S.

PY - 1990/3

Y1 - 1990/3

N2 - A recombinant DNA construct containing the neo gene controlled by a promoter from the Rous sarcoma virus (RSV) was microinjected into newly fertilized, dechorionated goldfish (Carassius auratus) eggs. The neo gene confers resistance to the neomycin analog drug G-418. Results of Southern blot analyses were consistent with incorporation of single or multiple copies of the gene into the genomic DNA of one examined fish. Evidence of neo mRNA in RNA dot-blot analysis indicated that the RSV promoter had initiated transcription within a piscine genome. The utility of the neo gene as a selectable marker for transgenic fish was evaluated by G-418 selection on newly hatched and juvenile fish but proved inconclusive. Reasons for the discrepancy between neo expression and G-418 selection results are discussed.

AB - A recombinant DNA construct containing the neo gene controlled by a promoter from the Rous sarcoma virus (RSV) was microinjected into newly fertilized, dechorionated goldfish (Carassius auratus) eggs. The neo gene confers resistance to the neomycin analog drug G-418. Results of Southern blot analyses were consistent with incorporation of single or multiple copies of the gene into the genomic DNA of one examined fish. Evidence of neo mRNA in RNA dot-blot analysis indicated that the RSV promoter had initiated transcription within a piscine genome. The utility of the neo gene as a selectable marker for transgenic fish was evaluated by G-418 selection on newly hatched and juvenile fish but proved inconclusive. Reasons for the discrepancy between neo expression and G-418 selection results are discussed.

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JO - Aquaculture

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ER -

Transfer of the gene for neomycin resistance into goldfish, Carassius auratus

Abstract

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