Transcriptome of channel catfish (Ictalurus punctatus): Initial analysis of genes and expression profiles of the head kidney

D. Cao, A. Kocabas, Z. Ju, A. Karsi, P. Li, A. Patterson, Z. Liu

Research output: Contribution to journalArticle

62 Scopus citations

Abstract

Analysis of expressed sequence tags (ESTs) is an efficient approach for gene discovery, expression profiling, and development of resources useful for functional genomics studies. As part of the transcriptome analysis in channel catfish (Ictalurus punctatus), we have conducted EST analysis using a cDNA library made from the head kidney. We analysed 2228 EST clones. Orthologues were established for 1495 (67.1%) clones representing 748 genes, of which 545 (36.5%) clones were singletons. The remaining 733 (32.9%) clones represent unknown gene clones, for which the number of genes has not yet been determined.

Original languageEnglish (US)
Pages (from-to)169-188
Number of pages20
JournalAnimal Genetics
Volume32
Issue number4
DOIs
StatePublished - 2001

Keywords

  • CDNA
  • Expressed sequence tag (EST)
  • Functional genomics
  • Gene expression
  • Mapping
  • Marker
  • Microarray

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Genetics

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