Transcriptome of channel catfish (Ictalurus punctatus): Initial analysis of genes and expression profiles of the head kidney

D. Cao; A. Kocabas; Z. Ju; A. Karsi; P. Li; A. Patterson; Z. Liu

doi:10.1046/j.1365-2052.2001.00753.x

Transcriptome of channel catfish (Ictalurus punctatus): Initial analysis of genes and expression profiles of the head kidney

D. Cao, A. Kocabas, Z. Ju, A. Karsi, P. Li, A. Patterson, Z. Liu

Research output: Contribution to journal › Article › peer-review

64 Scopus citations

Abstract

Analysis of expressed sequence tags (ESTs) is an efficient approach for gene discovery, expression profiling, and development of resources useful for functional genomics studies. As part of the transcriptome analysis in channel catfish (Ictalurus punctatus), we have conducted EST analysis using a cDNA library made from the head kidney. We analysed 2228 EST clones. Orthologues were established for 1495 (67.1%) clones representing 748 genes, of which 545 (36.5%) clones were singletons. The remaining 733 (32.9%) clones represent unknown gene clones, for which the number of genes has not yet been determined.

Original language	English (US)
Pages (from-to)	169-188
Number of pages	20
Journal	Animal Genetics
Volume	32
Issue number	4
DOIs	https://doi.org/10.1046/j.1365-2052.2001.00753.x
State	Published - 2001
Externally published	Yes

Keywords

CDNA
Expressed sequence tag (EST)
Functional genomics
Gene expression
Mapping
Marker
Microarray

ASJC Scopus subject areas

Animal Science and Zoology
Genetics

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10.1046/j.1365-2052.2001.00753.x

Cite this

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abstract = "Analysis of expressed sequence tags (ESTs) is an efficient approach for gene discovery, expression profiling, and development of resources useful for functional genomics studies. As part of the transcriptome analysis in channel catfish (Ictalurus punctatus), we have conducted EST analysis using a cDNA library made from the head kidney. We analysed 2228 EST clones. Orthologues were established for 1495 (67.1%) clones representing 748 genes, of which 545 (36.5%) clones were singletons. The remaining 733 (32.9%) clones represent unknown gene clones, for which the number of genes has not yet been determined.",

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T2 - Initial analysis of genes and expression profiles of the head kidney

AU - Cao, D.

AU - Kocabas, A.

AU - Ju, Z.

AU - Karsi, A.

AU - Li, P.

AU - Patterson, A.

AU - Liu, Z.

PY - 2001

Y1 - 2001

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AB - Analysis of expressed sequence tags (ESTs) is an efficient approach for gene discovery, expression profiling, and development of resources useful for functional genomics studies. As part of the transcriptome analysis in channel catfish (Ictalurus punctatus), we have conducted EST analysis using a cDNA library made from the head kidney. We analysed 2228 EST clones. Orthologues were established for 1495 (67.1%) clones representing 748 genes, of which 545 (36.5%) clones were singletons. The remaining 733 (32.9%) clones represent unknown gene clones, for which the number of genes has not yet been determined.

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KW - Functional genomics

KW - Gene expression

KW - Mapping

KW - Marker

KW - Microarray

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Transcriptome of channel catfish (Ictalurus punctatus): Initial analysis of genes and expression profiles of the head kidney

Abstract

Keywords

ASJC Scopus subject areas

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