Transcriptional regulation of hypoxia inducible factors alpha (HIF-α) and their inhibiting factor (FIH-1) of channel catfish (ictalurus punctatus) under hypoxia

Xin Geng, Jianbin Feng, Shikai Liu, Yaping Wang, Covadonga Arias, Zhanjiang Liu

Research output: Contribution to journalArticle

45 Scopus citations

Abstract

Hypoxia inducible factors (HIFs) are considered to be the master switch of oxygen-dependent gene expression with mammalian species. In most cases, regulation of HIF has been believed at posttranslational levels. However, little is known of HIF regulation in channel catfish, a species highly tolerant to low oxygen condition. Here we report the identification and characterization of HIF-1α, HIF-2αa, HIF-2αb, HIF-3α, and FIH-1 genes, and their mRNA expression under hypoxia conditions. The transcripts of the five genes were found to be regulated temporally and spatially after low oxygen challenge, suggesting regulation of HIF-α genes at pre-translational levels. In most tissues, the HIF-α mRNAs were down-regulated 1.5. h but up-regulated 5. h after hypoxia treatment. Of these HIF-α mRNAs, the expression of HIF-3α mRNA was induced in the most dramatic fashion, both in the speed of induction and the extent of induction, compared to HIF-1α and HIF-2α genes, suggesting its importance in responses to hypoxia.

Original languageEnglish (US)
Pages (from-to)38-50
Number of pages13
JournalComparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
Volume169
Issue number1
DOIs
StatePublished - Mar 2014
Externally publishedYes

Keywords

  • FIH-1
  • Fish
  • Gene
  • HIF
  • Hypoxia

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Molecular Biology

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