Time resolved calcium microdomains and synaptic transmission

R. Llinás; M. Sugimori; R. B. Silver

doi:10.1016/0928-4257(96)80554-7

Time resolved calcium microdomains and synaptic transmission

R. Llinás, M. Sugimori, R. B. Silver

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

The time course for the calcium entry that triggers release was studied at the squid giant synapse by imaging light emission from n-aequorin-J intracellularly injected into the presynaptic terminal. The imaging utilized a video system capable of acquiring 4000 frames per second. The results indicate that the calcium entry triggered by action potentials reaches a peak within 200 microseconds and has an overall duration of close to 800 microseconds, closely matching the duration of the presynaptic calcium current determined by voltage clamp results under similar conditions.

Original language	English (US)
Pages (from-to)	77-81
Number of pages	5
Journal	Journal of Physiology - Paris
Volume	89
Issue number	2
DOIs	https://doi.org/10.1016/0928-4257(96)80554-7
State	Published - 1995
Externally published	Yes

Keywords

imaging
light emission
n-aequorin-J
squid giant synapse

ASJC Scopus subject areas

General Neuroscience
Physiology (medical)

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10.1016/0928-4257(96)80554-7

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AU - Llinás, R.

AU - Sugimori, M.

AU - Silver, R. B.

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AB - The time course for the calcium entry that triggers release was studied at the squid giant synapse by imaging light emission from n-aequorin-J intracellularly injected into the presynaptic terminal. The imaging utilized a video system capable of acquiring 4000 frames per second. The results indicate that the calcium entry triggered by action potentials reaches a peak within 200 microseconds and has an overall duration of close to 800 microseconds, closely matching the duration of the presynaptic calcium current determined by voltage clamp results under similar conditions.

KW - imaging

KW - light emission

KW - n-aequorin-J

KW - squid giant synapse

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Time resolved calcium microdomains and synaptic transmission

Abstract

Keywords

ASJC Scopus subject areas

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