Three-dimensional reconstruction of large tissue volumes from scanning laser confocal microscopy

M. A. Slamani, A. Krol, J. Beaumont, R. L. Price, I. L. Coman, E. D. Lipson

Research output: Contribution to journalConference article

2 Scopus citations

Abstract

Phase correlation is applied to the mosaicing of confocal scanning laser microscopy data. A large specimen (i.e., a murine heart) is cut into a number of individual sections with appropriate thickness. The sections are scanned horizontally and vertically to produce tiles of a 3D volume. Image processing based on phase correlation is used to rebuild the 3D volume and stitch the tiles together. Specifically, 2D registration of in-plane tiles and 3D alignment of optical slices within a given physical section are performed. The approach and performance are presented in this paper along with examples.

Original languageEnglish (US)
Pages (from-to)1972-1979
Number of pages8
JournalProceedings of SPIE - The International Society for Optical Engineering
Volume5370 III
DOIs
StatePublished - Oct 27 2004
EventProgress in Biomedical Optics and Imaging - Medical Imaging 2004: Imaging Processing - San Diego, CA, United States
Duration: Feb 16 2004Feb 19 2004

Keywords

  • Confocal scanning laser microscopy
  • Mosaic
  • Phase correlation

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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