The syncytial Drosophila embryo as a mechanically excitable medium.

Timon Idema, Julien O. Dubuis, Louis Kang, M. Lisa Manning, Philip C. Nelson, Tom C. Lubensky, Andrea J. Liu

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25 Scopus citations

Abstract

Mitosis in the early syncytial Drosophila embryo is highly correlated in space and time, as manifested in mitotic wavefronts that propagate across the embryo. In this paper we investigate the idea that the embryo can be considered a mechanically-excitable medium, and that mitotic wavefronts can be understood as nonlinear wavefronts that propagate through this medium. We study the wavefronts via both image analysis of confocal microscopy videos and theoretical models. We find that the mitotic waves travel across the embryo at a well-defined speed that decreases with replication cycle. We find two markers of the wavefront in each cycle, corresponding to the onsets of metaphase and anaphase. Each of these onsets is followed by displacements of the nuclei that obey the same wavefront pattern. To understand the mitotic wavefronts theoretically we analyze wavefront propagation in excitable media. We study two classes of models, one with biochemical signaling and one with mechanical signaling. We find that the dependence of wavefront speed on cycle number is most naturally explained by mechanical signaling, and that the entire process suggests a scenario in which biochemical and mechanical signaling are coupled.

Original languageEnglish (US)
JournalPloS one
Volume8
Issue number10
DOIs
StatePublished - 2013

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ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Idema, T., Dubuis, J. O., Kang, L., Manning, M. L., Nelson, P. C., Lubensky, T. C., & Liu, A. J. (2013). The syncytial Drosophila embryo as a mechanically excitable medium. PloS one, 8(10). https://doi.org/10.1371/journal.pone.0077216