TY - JOUR
T1 - The Sex-lethal gene of drosophila
T2 - DNA alterations associated with sex-specific lethal mutations
AU - Maine, Eleanor M.
AU - Salz, Helen K.
AU - Cline, Thomas W.
AU - Schedl, Paul
N1 - Funding Information:
We thank J. Belote and T. Kaufman for providing some of the stocks used in this study. We thank E. Frei for providing us with his cosmid library. We also acknowledge N. Chapman for isolation of Sx/““l.fPa and N. Lyons and M. Engel for technical assistance in the genetic analyses. This work was supported by grants from NIH and March of Dimes to T. C., P S., and Princeton University Department of Biology.
PY - 1985/12
Y1 - 1985/12
N2 - Genomic DNA encoding Sex-lethal, a developmental switch gene in Drosophila melanogaster that regulates sex determination and dosage compensation has been isolated. Wild-type DNA sequence organization of the gene has been compared at the restriction level with those of 17 female-specific, loss-of-function and five male-specific, gain-of-function mutant alleles. DNA lesions associated with 12 of these mutations delimit an 11 kb DNA region that is necessary for proper Sex-lethal function in females. Males who are deleted for this region are both viable and fertile. Loss-of-function alleles are associated with gross DNA alterations as well as true point mutations; the former are located throughout the region. In contrast, all five gain-of-function alleles are associated with DNA insertions that are clustered within a 1 kb portion of the Sxl gene region.
AB - Genomic DNA encoding Sex-lethal, a developmental switch gene in Drosophila melanogaster that regulates sex determination and dosage compensation has been isolated. Wild-type DNA sequence organization of the gene has been compared at the restriction level with those of 17 female-specific, loss-of-function and five male-specific, gain-of-function mutant alleles. DNA lesions associated with 12 of these mutations delimit an 11 kb DNA region that is necessary for proper Sex-lethal function in females. Males who are deleted for this region are both viable and fertile. Loss-of-function alleles are associated with gross DNA alterations as well as true point mutations; the former are located throughout the region. In contrast, all five gain-of-function alleles are associated with DNA insertions that are clustered within a 1 kb portion of the Sxl gene region.
UR - http://www.scopus.com/inward/record.url?scp=0022378518&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0022378518&partnerID=8YFLogxK
U2 - 10.1016/0092-8674(85)90181-3
DO - 10.1016/0092-8674(85)90181-3
M3 - Article
C2 - 3000609
AN - SCOPUS:0022378518
SN - 0092-8674
VL - 43
SP - 521
EP - 529
JO - Cell
JF - Cell
IS - 2 PART 1
ER -