The mechanisms of acid-base and ionoregulation in the freshwater rainbow trout during environmental hyperoxia and subsequent normoxia. III. Branchial exchanges

Chris M. Wood, Michele G. Wheatly, Helve Hobe

Research output: Contribution to journalArticle

51 Scopus citations

Abstract

Fluxes of both acidic equivalents (JnetH+) and electrolytes across the gills were continuously monitored in the freshwater rainbow trout (Salmo gairdneri) during 24 h normoxia (PIO2 = 120-150 torr; control), 72 h hyperoxia (PIO2 = 500-600 torr), and 24 h return to normoxia. A highly negative JnetH+ (i.e., excretion) was responsible for over 90% of the compensation of respiratory acidosis induced by hyperoxia in the whole animal. Similarly, a highly positive JnetH+ (i.e., uptake)_accounted for virtually all the compensation of metabolic alkalosis induced by normoxic recovery. Hyperoxia was associated with a small net gain of Na+ and large net losses of Cl- at the gills, while normoxic recovery was associated with large net losses of Na+ and net gains of Cl-, effects reflected in ECF composition. Unidirectional flux analyses with radiotracers (22Na, 36Cl) demonstrated that these net flux alterations resulted from rapid and complex changes in both influx and efflux components such that the difference between JnetNa+ and JnetCl- was stoichiometrically equivalent to JnetH+. The results support the concept that Na+vs acidic equivalent (H+, NH+4) and Cl-vs basic equivalent (HCO3-, OH-) exchanges at the gill are dynamically adjusted in order to correct internal acid-base disturbances.

Original languageEnglish (US)
Pages (from-to)175-192
Number of pages18
JournalRespiration Physiology
Volume55
Issue number2
DOIs
StatePublished - Feb 1984
Externally publishedYes

Keywords

  • Acid-base balance
  • Branchial fluxes
  • Chloride exchange
  • Gills
  • Hyperoxia
  • Ionoregulation
  • Salmo gairdneri
  • Sodium exchange

ASJC Scopus subject areas

  • Physiology
  • Pulmonary and Respiratory Medicine

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