Antibodies previously shown to inhibit vectorial proton translocation through cytochrome oxidase vesicles were converted to F(ab′)2 and Fab′. Neither fragment inhibited proton pumping, although binding capacity was present. However, when a surrogate Fc was added to F(ab′)2, inhibition of proton translocation was restored; indicating that the inhibition is due to steric hindrance. These results provide insight into mechanisms of energy transduction by oxidase.
|Original language||English (US)|
|Number of pages||7|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Apr 16 1984|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology