The mechanism of antibody inhibition of proton pumping by cytochrome oxidase vesicles

Jo A. Freedman, Raymond L. Bratcher, Samuel H.P. Chan

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Antibodies previously shown to inhibit vectorial proton translocation through cytochrome oxidase vesicles were converted to F(ab′)2 and Fab′. Neither fragment inhibited proton pumping, although binding capacity was present. However, when a surrogate Fc was added to F(ab′)2, inhibition of proton translocation was restored; indicating that the inhibition is due to steric hindrance. These results provide insight into mechanisms of energy transduction by oxidase.

Original languageEnglish (US)
Pages (from-to)52-58
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume120
Issue number1
DOIs
StatePublished - Apr 16 1984

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'The mechanism of antibody inhibition of proton pumping by cytochrome oxidase vesicles'. Together they form a unique fingerprint.

Cite this