TY - JOUR
T1 - The mechanism of antibody inhibition of proton pumping by cytochrome oxidase vesicles
AU - Freedman, Jo A.
AU - Bratcher, Raymond L.
AU - Chan, Samuel H.P.
PY - 1984/4/16
Y1 - 1984/4/16
N2 - Antibodies previously shown to inhibit vectorial proton translocation through cytochrome oxidase vesicles were converted to F(ab′)2 and Fab′. Neither fragment inhibited proton pumping, although binding capacity was present. However, when a surrogate Fc was added to F(ab′)2, inhibition of proton translocation was restored; indicating that the inhibition is due to steric hindrance. These results provide insight into mechanisms of energy transduction by oxidase.
AB - Antibodies previously shown to inhibit vectorial proton translocation through cytochrome oxidase vesicles were converted to F(ab′)2 and Fab′. Neither fragment inhibited proton pumping, although binding capacity was present. However, when a surrogate Fc was added to F(ab′)2, inhibition of proton translocation was restored; indicating that the inhibition is due to steric hindrance. These results provide insight into mechanisms of energy transduction by oxidase.
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U2 - 10.1016/0006-291X(84)91412-8
DO - 10.1016/0006-291X(84)91412-8
M3 - Article
C2 - 6324795
AN - SCOPUS:0021765207
SN - 0006-291X
VL - 120
SP - 52
EP - 58
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -