TY - JOUR
T1 - The iron complexes of bleomycin and tallysomycin
AU - Dabrowiak, James C.
AU - Greenaway, Frederick T.
AU - Santillo, Frank S.
AU - Crooke, S. T.
PY - 1979/12/14
Y1 - 1979/12/14
N2 - Using uv-visible absorption, epr, electrochemistry, and 13C nmr, the Fe(II) and Fe(III) binding sites of the antitumor antibiotics bleomycin and tallysomycin have been located. Both drugs appear to utilize the amine-pyrimidine-imidazole region for iron binding. The ligating atoms of the drugs for Fe(II) and Fe(III) are dependent for iron and the presence of buffer ions. The ligation of the pyrimidine moiety has been determined under a variety of experimental conditions and correlated with epr observation of high and low spin forms of Fe(III). The results indicate that the displacement of some of the ligating atoms does not inhibit the action of the iron-drug complex.
AB - Using uv-visible absorption, epr, electrochemistry, and 13C nmr, the Fe(II) and Fe(III) binding sites of the antitumor antibiotics bleomycin and tallysomycin have been located. Both drugs appear to utilize the amine-pyrimidine-imidazole region for iron binding. The ligating atoms of the drugs for Fe(II) and Fe(III) are dependent for iron and the presence of buffer ions. The ligation of the pyrimidine moiety has been determined under a variety of experimental conditions and correlated with epr observation of high and low spin forms of Fe(III). The results indicate that the displacement of some of the ligating atoms does not inhibit the action of the iron-drug complex.
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U2 - 10.1016/0006-291X(79)91940-5
DO - 10.1016/0006-291X(79)91940-5
M3 - Article
C2 - 93477
AN - SCOPUS:0018634879
SN - 0006-291X
VL - 91
SP - 721
EP - 729
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -