The ionic basis of the membrane potential in the early Asterias embryo

Joseph T. Tupper

doi:10.1016/0012-1606(72)90067-X

The ionic basis of the membrane potential in the early Asterias embryo

Joseph T. Tupper

Department of Biology

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

The ionic basis of the resting potential in the fertilized Asterias embryo has been investigated. The response of the membrane potential (as determined by intracellular microelectrodes) to changes in external K concentration was stable and reversible up to 200 mM K. The time course and magnitude of the response observed upon changes in external K is essentially independent of the concentration of external Cl. There is a significant effect of Na ion on the membrane potential, especially at low external K. The response of the membrane potential to changes in external K could be predicted by a form of the constant field equation. Plots of e^{VF RT} versus external K, in conjunction with the constant field equation, allow an estimate of the internal K concentration and the ratio of Na to K permeability ( P_Na P_K). These plots indicate at this stage of development an internal K concentration of approximately 200 mM and a P_Na P_K of 0.03.

Original language	English (US)
Pages (from-to)	273-282
Number of pages	10
Journal	Developmental Biology
Volume	29
Issue number	3
DOIs	https://doi.org/10.1016/0012-1606(72)90067-X
State	Published - Nov 1972

ASJC Scopus subject areas

Molecular Biology
Developmental Biology
Cell Biology

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10.1016/0012-1606(72)90067-X

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title = "The ionic basis of the membrane potential in the early Asterias embryo",

abstract = "The ionic basis of the resting potential in the fertilized Asterias embryo has been investigated. The response of the membrane potential (as determined by intracellular microelectrodes) to changes in external K concentration was stable and reversible up to 200 mM K. The time course and magnitude of the response observed upon changes in external K is essentially independent of the concentration of external Cl. There is a significant effect of Na ion on the membrane potential, especially at low external K. The response of the membrane potential to changes in external K could be predicted by a form of the constant field equation. Plots of e VF RT versus external K, in conjunction with the constant field equation, allow an estimate of the internal K concentration and the ratio of Na to K permeability ( PNa PK). These plots indicate at this stage of development an internal K concentration of approximately 200 mM and a PNa PK of 0.03.",

author = "Tupper, {Joseph T.}",

note = "Funding Information: The author greatly appreciates the helpful suggestions of Dr. Philip B. Dunham. This work was supported by Grant No. VC-37 from the American Cancer Society and by the Syracuse University Office of Sponsored Programs. Portions of this work have appeared in abstract form elsewhere (Tupper and Saunders, 1971; Tupper and Powers, 1972).",

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N1 - Funding Information: The author greatly appreciates the helpful suggestions of Dr. Philip B. Dunham. This work was supported by Grant No. VC-37 from the American Cancer Society and by the Syracuse University Office of Sponsored Programs. Portions of this work have appeared in abstract form elsewhere (Tupper and Saunders, 1971; Tupper and Powers, 1972).

PY - 1972/11

Y1 - 1972/11

N2 - The ionic basis of the resting potential in the fertilized Asterias embryo has been investigated. The response of the membrane potential (as determined by intracellular microelectrodes) to changes in external K concentration was stable and reversible up to 200 mM K. The time course and magnitude of the response observed upon changes in external K is essentially independent of the concentration of external Cl. There is a significant effect of Na ion on the membrane potential, especially at low external K. The response of the membrane potential to changes in external K could be predicted by a form of the constant field equation. Plots of e VF RT versus external K, in conjunction with the constant field equation, allow an estimate of the internal K concentration and the ratio of Na to K permeability ( PNa PK). These plots indicate at this stage of development an internal K concentration of approximately 200 mM and a PNa PK of 0.03.

AB - The ionic basis of the resting potential in the fertilized Asterias embryo has been investigated. The response of the membrane potential (as determined by intracellular microelectrodes) to changes in external K concentration was stable and reversible up to 200 mM K. The time course and magnitude of the response observed upon changes in external K is essentially independent of the concentration of external Cl. There is a significant effect of Na ion on the membrane potential, especially at low external K. The response of the membrane potential to changes in external K could be predicted by a form of the constant field equation. Plots of e VF RT versus external K, in conjunction with the constant field equation, allow an estimate of the internal K concentration and the ratio of Na to K permeability ( PNa PK). These plots indicate at this stage of development an internal K concentration of approximately 200 mM and a PNa PK of 0.03.

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ER -

The ionic basis of the membrane potential in the early Asterias embryo

Abstract

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