The ionic basis of the membrane potential in the early Asterias embryo

Joseph T. Tupper

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The ionic basis of the resting potential in the fertilized Asterias embryo has been investigated. The response of the membrane potential (as determined by intracellular microelectrodes) to changes in external K concentration was stable and reversible up to 200 mM K. The time course and magnitude of the response observed upon changes in external K is essentially independent of the concentration of external Cl. There is a significant effect of Na ion on the membrane potential, especially at low external K. The response of the membrane potential to changes in external K could be predicted by a form of the constant field equation. Plots of e VF RT versus external K, in conjunction with the constant field equation, allow an estimate of the internal K concentration and the ratio of Na to K permeability ( PNa PK). These plots indicate at this stage of development an internal K concentration of approximately 200 mM and a PNa PK of 0.03.

Original languageEnglish (US)
Pages (from-to)273-282
Number of pages10
JournalDevelopmental Biology
Volume29
Issue number3
DOIs
StatePublished - 1972

Fingerprint

Asterias
Membrane Potentials
Embryonic Structures
Microelectrodes
Permeability
Ions

ASJC Scopus subject areas

  • Developmental Biology

Cite this

The ionic basis of the membrane potential in the early Asterias embryo. / Tupper, Joseph T.

In: Developmental Biology, Vol. 29, No. 3, 1972, p. 273-282.

Research output: Contribution to journalArticle

Tupper, Joseph T. / The ionic basis of the membrane potential in the early Asterias embryo. In: Developmental Biology. 1972 ; Vol. 29, No. 3. pp. 273-282.
@article{6c1a8611ff524c93baa3a2f819b1fcd1,
title = "The ionic basis of the membrane potential in the early Asterias embryo",
abstract = "The ionic basis of the resting potential in the fertilized Asterias embryo has been investigated. The response of the membrane potential (as determined by intracellular microelectrodes) to changes in external K concentration was stable and reversible up to 200 mM K. The time course and magnitude of the response observed upon changes in external K is essentially independent of the concentration of external Cl. There is a significant effect of Na ion on the membrane potential, especially at low external K. The response of the membrane potential to changes in external K could be predicted by a form of the constant field equation. Plots of e VF RT versus external K, in conjunction with the constant field equation, allow an estimate of the internal K concentration and the ratio of Na to K permeability ( PNa PK). These plots indicate at this stage of development an internal K concentration of approximately 200 mM and a PNa PK of 0.03.",
author = "Tupper, {Joseph T.}",
year = "1972",
doi = "10.1016/0012-1606(72)90067-X",
language = "English (US)",
volume = "29",
pages = "273--282",
journal = "Developmental Biology",
issn = "0012-1606",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - The ionic basis of the membrane potential in the early Asterias embryo

AU - Tupper, Joseph T.

PY - 1972

Y1 - 1972

N2 - The ionic basis of the resting potential in the fertilized Asterias embryo has been investigated. The response of the membrane potential (as determined by intracellular microelectrodes) to changes in external K concentration was stable and reversible up to 200 mM K. The time course and magnitude of the response observed upon changes in external K is essentially independent of the concentration of external Cl. There is a significant effect of Na ion on the membrane potential, especially at low external K. The response of the membrane potential to changes in external K could be predicted by a form of the constant field equation. Plots of e VF RT versus external K, in conjunction with the constant field equation, allow an estimate of the internal K concentration and the ratio of Na to K permeability ( PNa PK). These plots indicate at this stage of development an internal K concentration of approximately 200 mM and a PNa PK of 0.03.

AB - The ionic basis of the resting potential in the fertilized Asterias embryo has been investigated. The response of the membrane potential (as determined by intracellular microelectrodes) to changes in external K concentration was stable and reversible up to 200 mM K. The time course and magnitude of the response observed upon changes in external K is essentially independent of the concentration of external Cl. There is a significant effect of Na ion on the membrane potential, especially at low external K. The response of the membrane potential to changes in external K could be predicted by a form of the constant field equation. Plots of e VF RT versus external K, in conjunction with the constant field equation, allow an estimate of the internal K concentration and the ratio of Na to K permeability ( PNa PK). These plots indicate at this stage of development an internal K concentration of approximately 200 mM and a PNa PK of 0.03.

UR - http://www.scopus.com/inward/record.url?scp=0015429811&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0015429811&partnerID=8YFLogxK

U2 - 10.1016/0012-1606(72)90067-X

DO - 10.1016/0012-1606(72)90067-X

M3 - Article

C2 - 4652269

AN - SCOPUS:0015429811

VL - 29

SP - 273

EP - 282

JO - Developmental Biology

JF - Developmental Biology

SN - 0012-1606

IS - 3

ER -