The effect of specific antibodies on oxygen uptake and H+ pumping by cytochrome c oxidase vesicles

Samuel H.P. Chan, Jo A. Freedman

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Antibodies to solubilized cytochrome c oxidse and to subunit III were incubated with liposomal oxidase. In oxygen uptake experiments, the inhibiting effects on RCI of anti-oxidase (primarily anti- subunits II and IV) and anti-III were by different mechanisms: the former, by inhibiting the uncoupled rate; the latter, by stimulating the coupled rate. In experiments with H+ translocation, anti-oxidase was without effect, while anti-III was a potent inhibitor of proton pumping. These results are conclusive evidence for redox-linked proton extrusion from the vesicles by the oxidase (and its subunit III).

Original languageEnglish (US)
Pages (from-to)344-348
Number of pages5
JournalFEBS Letters
Volume162
Issue number2
DOIs
StatePublished - Oct 17 1983

Keywords

  • Antibody
  • Chemiosmosis
  • Cytochrome c oxidase
  • Proton pumping
  • Reconstituted vesicle

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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