The E6 gene of human papillomavirus type 16 is sufficient for transformation of baby rat kidney cells in cotransfection with activated Ha- ras

Zhanjiang Liu; Jyotsna Ghai; Ronald S. Ostrow; Ronald C. McGlennen; Anthony J. Faras

doi:10.1006/viro.1994.1306

The E6 gene of human papillomavirus type 16 is sufficient for transformation of baby rat kidney cells in cotransfection with activated Ha- ras

Zhanjiang Liu, Jyotsna Ghai, Ronald S. Ostrow, Ronald C. McGlennen, Anthony J. Faras

Research output: Contribution to journal › Article › peer-review

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Abstract

The transforming potential of the E6 open reading frame (ORF) of the human papillomavirus type 16 was investigated with transformation assays in cotransfections with an activated ras gene. The E6 ORF driven by the heterologous CMV promoter could fully transform baby rat kidney cells (BRK) in cooperation with ras. The transformed cells grew in soft agar and induced tumors in athymic nude mice. The E6 ORF with mutations at the splicing donor site, which only encodes the full length E6 but not E6*s, could also fully transform the BRK cells at a similar efficiency as the wild-type E6 ORF, indicating that the full-length E6 was sufficient for the transformed phenotype.

Original language	English (US)
Article number	71306
Pages (from-to)	388-396
Number of pages	9
Journal	Virology
Volume	201
Issue number	2
DOIs	https://doi.org/10.1006/viro.1994.1306
State	Published - Jun 1994
Externally published	Yes

ASJC Scopus subject areas

Virology

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title = "The E6 gene of human papillomavirus type 16 is sufficient for transformation of baby rat kidney cells in cotransfection with activated Ha- ras",

abstract = "The transforming potential of the E6 open reading frame (ORF) of the human papillomavirus type 16 was investigated with transformation assays in cotransfections with an activated ras gene. The E6 ORF driven by the heterologous CMV promoter could fully transform baby rat kidney cells (BRK) in cooperation with ras. The transformed cells grew in soft agar and induced tumors in athymic nude mice. The E6 ORF with mutations at the splicing donor site, which only encodes the full length E6 but not E6*s, could also fully transform the BRK cells at a similar efficiency as the wild-type E6 ORF, indicating that the full-length E6 was sufficient for the transformed phenotype.",

author = "Zhanjiang Liu and Jyotsna Ghai and Ostrow, {Ronald S.} and McGlennen, {Ronald C.} and Faras, {Anthony J.}",

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AU - Ghai, Jyotsna

AU - Ostrow, Ronald S.

AU - McGlennen, Ronald C.

AU - Faras, Anthony J.

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AB - The transforming potential of the E6 open reading frame (ORF) of the human papillomavirus type 16 was investigated with transformation assays in cotransfections with an activated ras gene. The E6 ORF driven by the heterologous CMV promoter could fully transform baby rat kidney cells (BRK) in cooperation with ras. The transformed cells grew in soft agar and induced tumors in athymic nude mice. The E6 ORF with mutations at the splicing donor site, which only encodes the full length E6 but not E6*s, could also fully transform the BRK cells at a similar efficiency as the wild-type E6 ORF, indicating that the full-length E6 was sufficient for the transformed phenotype.

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The E6 gene of human papillomavirus type 16 is sufficient for transformation of baby rat kidney cells in cotransfection with activated Ha- ras

Abstract

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