TY - JOUR
T1 - The Drosophila melanogaster sperm proteome-II (DmSP-II)
AU - Wasbrough, Elizabeth R.
AU - Dorus, Steve
AU - Hester, Svenja
AU - Howard-Murkin, Julie
AU - Lilley, Kathryn
AU - Wilkin, Elaine
AU - Polpitiya, Ashoka
AU - Petritis, Konstantinos
AU - Karr, Timothy L.
N1 - Funding Information:
This research was supported by a Wolfson Merit Award from the Royal Society and the Biodesign Institute, Arizona State University (TLK), and by a Ruth L. Kirschstein National Research Service Award (National Institutes of Health) and an Academic Research Fellowship from the Research Council of the United Kingdom (SD).
PY - 2010/10/10
Y1 - 2010/10/10
N2 - Advances in mass spectrometry technology, high-throughput proteomics and genome annotations have resulted in significant increases in our molecular understanding of sperm composition. Using improved separation and detection methods and an updated genome annotation, a re-analysis of the Drosophila melanogaster sperm proteome (DmSP) has resulted in the identification of 956 sperm proteins. Comparative analysis with our previous proteomic dataset revealed 766 new proteins and an updated sperm proteome containing a total of 1108 proteins, termed the DmSP-II. This expanded dataset includes additional proteins with predicted sperm functions and confirms previous findings concerning the genomic organization of sperm loci. Bioinformatic and protein network analyses demonstrated high quality and reproducibility of proteome coverage across three replicate mass spectrometry runs. The use of whole-cell proteomics in conjunction with characterized phenotypes, functional annotations and pathway information has advanced our systems level understanding of sperm proteome functional networks.
AB - Advances in mass spectrometry technology, high-throughput proteomics and genome annotations have resulted in significant increases in our molecular understanding of sperm composition. Using improved separation and detection methods and an updated genome annotation, a re-analysis of the Drosophila melanogaster sperm proteome (DmSP) has resulted in the identification of 956 sperm proteins. Comparative analysis with our previous proteomic dataset revealed 766 new proteins and an updated sperm proteome containing a total of 1108 proteins, termed the DmSP-II. This expanded dataset includes additional proteins with predicted sperm functions and confirms previous findings concerning the genomic organization of sperm loci. Bioinformatic and protein network analyses demonstrated high quality and reproducibility of proteome coverage across three replicate mass spectrometry runs. The use of whole-cell proteomics in conjunction with characterized phenotypes, functional annotations and pathway information has advanced our systems level understanding of sperm proteome functional networks.
KW - Evolution
KW - Fertility
KW - Genomics
KW - Shotgun proteomics
KW - Spermatogenesis
UR - http://www.scopus.com/inward/record.url?scp=77957243009&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77957243009&partnerID=8YFLogxK
U2 - 10.1016/j.jprot.2010.09.002
DO - 10.1016/j.jprot.2010.09.002
M3 - Article
C2 - 20833280
AN - SCOPUS:77957243009
SN - 1874-3919
VL - 73
SP - 2171
EP - 2185
JO - Journal of Proteomics
JF - Journal of Proteomics
IS - 11
ER -