The DPY-30 domain and its flanking sequence mediate the assembly and modulation of flagellar radial spoke complexes

Radhika Gopal, Kenneth W. Foster, Pinfen Yang

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

RIIa is known as the dimerization and docking (D/D) domain of the cyclic AMP (cAMP)-dependent protein kinase. However, numerous molecules, including radial spoke protein 2 (RSP2) in Chlamydomonas flagella, also contain an RIIa or a similar DPY-30 domain. To elucidate new roles of D/D domain-containing proteins, we investigated a panel of RSP2 mutants. An RSP2 mutant had paralyzed flagella defective in RSP2 and multiple subunits near the spokehead. New transgenic strains lacking only the DPY-30 domain in RSP2 were also paralyzed. In contrast, motility was restored in strains that lacked only RSP2's calmodulin-binding C-terminal region. These cells swam normally in dim light but could not maintain typical swimming trajectories under bright illumination. In both deletion transgenic strains, the subunits near the spokehead were restored, but their firm attachment to the spokestalk required the DPY-30 domain. We postulate that the DPY-30-helix dimer is a conserved two-prong linker, required for normal motility, organizing duplicated subunits in the radial spoke stalk and formation of a symmetrical spokehead. Further, the dispensable calmodulin-binding region appears to fine-tune the spokehead for regulation of "steering" motility in the green algae. Thus, in general, D/D domains may function to localize molecular modules for both the assembly and modulation of macromolecular complexes.

Original languageEnglish (US)
Pages (from-to)4012-4024
Number of pages13
JournalMolecular and Cellular Biology
Volume32
Issue number19
DOIs
StatePublished - Oct 2012

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Dimerization
Flagella
Mutant Proteins
Calmodulin
Macromolecular Substances
Chlamydomonas
Chlorophyta
Proteins
Cyclic AMP-Dependent Protein Kinases
Lighting
Light

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

The DPY-30 domain and its flanking sequence mediate the assembly and modulation of flagellar radial spoke complexes. / Gopal, Radhika; Foster, Kenneth W.; Yang, Pinfen.

In: Molecular and Cellular Biology, Vol. 32, No. 19, 10.2012, p. 4012-4024.

Research output: Contribution to journalArticle

Gopal, Radhika ; Foster, Kenneth W. ; Yang, Pinfen. / The DPY-30 domain and its flanking sequence mediate the assembly and modulation of flagellar radial spoke complexes. In: Molecular and Cellular Biology. 2012 ; Vol. 32, No. 19. pp. 4012-4024.
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