The chemokinome superfamily

II. The 64 CC chemokines in channel catfish and their involvement in disease and hypoxia responses

Qiang Fu, Yujia Yang, Chao Li, Qifan Zeng, Tao Zhou, Ning Li, Yang Liu, Yun Li, Xiaozhu Wang, Shikai Liu, Daoji Li, Zhanjian "John" Liu

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Chemokines are a superfamily of structurally related chemotactic cytokines exerting significant roles in regulating cell migration and activation. Based on the arrangement of the first four cysteine residues, they are classified into CC, CXC, C and CX3C subfamilies. In this study, a complete set of 64 CC chemokine ligand (CCL) genes was systematically identified, annotated, and characterized from the channel catfish genome. Extensive phylogenetic and comparative genomic analyses supported their annotations, allowing establishment of their orthologies, revealing fish-specific CC chemokines and the expansion of CC chemokines in the teleost genomes through lineage-specific tandem duplications. With 64 genes, the channel catfish genome harbors the largest numbers of CC chemokines among all the genomes characterized to date, however, they fall into 11 distinct CC chemokine groups. Analysis of gene expression after bacterial infections indicated that the CC chemokines were regulated in a gene-specific and time-dependent manner. While only one member of CCL19 (CCL19a.1) was significantly up-regulated after Edwardsiella ictaluri infection, all CCL19 members (CCL19a.1, CCL19a.2 and CCL19b) were significantly induced after Flavobacterium columnare infection. In addition, CCL19a.1, CCL19a.2 and CCL19b were also drastically up-regulated in ESC-susceptible fish, but not in resistant fish, suggesting potential significant roles of CCL19 in catfish immune responses. High expression levels of certain CC appeared to be correlated with susceptibility to diseases and intolerance to hypoxia.

Original languageEnglish (US)
Pages (from-to)97-108
Number of pages12
JournalDevelopmental and Comparative Immunology
Volume73
DOIs
StatePublished - Aug 1 2017
Externally publishedYes

Fingerprint

Ictaluridae
CC Chemokines
Genome
Fishes
Chemokines
Edwardsiella ictaluri
Genes
Flavobacterium
Catfishes
Disease Susceptibility
Infection
Bacterial Infections
Cell Movement
Cysteine
Hypoxia
Ligands
Gene Expression

Keywords

  • Chemokine
  • Disease resistance
  • Fish
  • Genome
  • Hypoxia
  • Innate immunity

ASJC Scopus subject areas

  • Immunology
  • Developmental Biology

Cite this

The chemokinome superfamily : II. The 64 CC chemokines in channel catfish and their involvement in disease and hypoxia responses. / Fu, Qiang; Yang, Yujia; Li, Chao; Zeng, Qifan; Zhou, Tao; Li, Ning; Liu, Yang; Li, Yun; Wang, Xiaozhu; Liu, Shikai; Li, Daoji; Liu, Zhanjian "John".

In: Developmental and Comparative Immunology, Vol. 73, 01.08.2017, p. 97-108.

Research output: Contribution to journalArticle

Fu, Qiang ; Yang, Yujia ; Li, Chao ; Zeng, Qifan ; Zhou, Tao ; Li, Ning ; Liu, Yang ; Li, Yun ; Wang, Xiaozhu ; Liu, Shikai ; Li, Daoji ; Liu, Zhanjian "John". / The chemokinome superfamily : II. The 64 CC chemokines in channel catfish and their involvement in disease and hypoxia responses. In: Developmental and Comparative Immunology. 2017 ; Vol. 73. pp. 97-108.
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