The Azotobacter vinelandii nifL-like gene: nucleotide sequence analysis and regulation of expression

Ramesh Raina, Umesh K. Bageshwar, H. K. Das

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The nucleotide sequence of the Azotobacter vinelandii nifL-like gene (Av-nifL) was determined. The 1.9 kb sequence shows an open reading frame (ORF) of 1557 by which encodes a polypeptide of 519 amino acids, with a calculated molecular weight of 57 793. Av-nifL has about 50 % homology with the Klebsiella pneumoniae nifL gene (Kp-nifL) at the nucleotide level and a little more than 52% homology at the amino acid level. The N-terminal regions show more homology than the C-terminal regions. As is the case in K. pneumoniae, Av-nifL is located just upstream of the A. vinelandii nifA gene (Av-nifA) and both genes constitute an operon. The expression of Av-nifL, however, seems to be independent of NtrA and NtrC. Furthermore, Av-nifL expression is not autogenously regulated by NifA, unlike the case in K. pneumoniae. The expression of an Av-nifL: lacZ fusion in A. vinelandii is inhibited by novobiocin and coumermycin A, which are inhibitors of DNA gyrase.

Original languageEnglish (US)
Pages (from-to)400-406
Number of pages7
JournalMGG Molecular & General Genetics
Volume237
Issue number3
DOIs
StatePublished - Mar 1993
Externally publishedYes

Fingerprint

Azotobacter vinelandii
Sequence Analysis
Genes
Klebsiella pneumoniae
Novobiocin
Topoisomerase II Inhibitors
Amino Acids
Gene Fusion
Operon
Open Reading Frames
Nucleotides
Molecular Weight
Gene Expression
Peptides

Keywords

  • Azotobacter vinelandii
  • DNA gyrase
  • nifL: lacZ fusion
  • ntrA
  • Promoter

ASJC Scopus subject areas

  • Genetics

Cite this

The Azotobacter vinelandii nifL-like gene : nucleotide sequence analysis and regulation of expression. / Raina, Ramesh; Bageshwar, Umesh K.; Das, H. K.

In: MGG Molecular & General Genetics, Vol. 237, No. 3, 03.1993, p. 400-406.

Research output: Contribution to journalArticle

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