The activation of platinum(II) antiproliferative drugs in carbonate medium evaluated by means of a DNA-biosensor

Mauro Ravera; Graziana Bagni; Marco Mascini; James C. Dabrowiak; Domenico Osella

doi:10.1016/j.jinorgbio.2007.03.017

The activation of platinum(II) antiproliferative drugs in carbonate medium evaluated by means of a DNA-biosensor

Mauro Ravera, Graziana Bagni, Marco Mascini, James C. Dabrowiak, Domenico Osella

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

We report on the binding of cisplatin, carboplatin and oxaliplatin to double-stranded DNA in two different (phosphate and carbonate) buffers, using an electrochemical DNA-biosensor. The propensity of the electrophilic agent produced by hydrolysis to interact with DNA was measured as a function of the decrease of guanine oxidation signal of the metal-DNA adduct immobilized on a screen-printed electrode, by using square wave voltammetry. The results obtained confirm that carbonate reacts with platinum drugs to form activated carbonato complexes, which are able to react readily with DNA.

Original language	English (US)
Pages (from-to)	1023-1027
Number of pages	5
Journal	Journal of Inorganic Biochemistry
Volume	101
Issue number	7
DOIs	https://doi.org/10.1016/j.jinorgbio.2007.03.017
State	Published - Jul 2007

Keywords

Carbonate buffer
DNA-biosensor
Platinum drugs

ASJC Scopus subject areas

Biochemistry
Inorganic Chemistry

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10.1016/j.jinorgbio.2007.03.017

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title = "The activation of platinum(II) antiproliferative drugs in carbonate medium evaluated by means of a DNA-biosensor",

abstract = "We report on the binding of cisplatin, carboplatin and oxaliplatin to double-stranded DNA in two different (phosphate and carbonate) buffers, using an electrochemical DNA-biosensor. The propensity of the electrophilic agent produced by hydrolysis to interact with DNA was measured as a function of the decrease of guanine oxidation signal of the metal-DNA adduct immobilized on a screen-printed electrode, by using square wave voltammetry. The results obtained confirm that carbonate reacts with platinum drugs to form activated carbonato complexes, which are able to react readily with DNA.",

keywords = "Carbonate buffer, DNA-biosensor, Platinum drugs",

author = "Mauro Ravera and Graziana Bagni and Marco Mascini and Dabrowiak, {James C.} and Domenico Osella",

note = "Funding Information: This work was financially supported by Regione Piemonte and ATF (Alessandria). The research was carried out within the framework of the European Cooperation COST D39 (Metallo-Drug Design and Action) and COST B16 action (Multidrug resistance reversal).",

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T1 - The activation of platinum(II) antiproliferative drugs in carbonate medium evaluated by means of a DNA-biosensor

AU - Ravera, Mauro

AU - Bagni, Graziana

AU - Mascini, Marco

AU - Dabrowiak, James C.

AU - Osella, Domenico

N1 - Funding Information: This work was financially supported by Regione Piemonte and ATF (Alessandria). The research was carried out within the framework of the European Cooperation COST D39 (Metallo-Drug Design and Action) and COST B16 action (Multidrug resistance reversal).

PY - 2007/7

Y1 - 2007/7

N2 - We report on the binding of cisplatin, carboplatin and oxaliplatin to double-stranded DNA in two different (phosphate and carbonate) buffers, using an electrochemical DNA-biosensor. The propensity of the electrophilic agent produced by hydrolysis to interact with DNA was measured as a function of the decrease of guanine oxidation signal of the metal-DNA adduct immobilized on a screen-printed electrode, by using square wave voltammetry. The results obtained confirm that carbonate reacts with platinum drugs to form activated carbonato complexes, which are able to react readily with DNA.

AB - We report on the binding of cisplatin, carboplatin and oxaliplatin to double-stranded DNA in two different (phosphate and carbonate) buffers, using an electrochemical DNA-biosensor. The propensity of the electrophilic agent produced by hydrolysis to interact with DNA was measured as a function of the decrease of guanine oxidation signal of the metal-DNA adduct immobilized on a screen-printed electrode, by using square wave voltammetry. The results obtained confirm that carbonate reacts with platinum drugs to form activated carbonato complexes, which are able to react readily with DNA.

KW - Carbonate buffer

KW - DNA-biosensor

KW - Platinum drugs

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The activation of platinum(II) antiproliferative drugs in carbonate medium evaluated by means of a DNA-biosensor

Abstract

Keywords

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