The activation of platinum(II) antiproliferative drugs in carbonate medium evaluated by means of a DNA-biosensor

Mauro Ravera, Graziana Bagni, Marco Mascini, James C. Dabrowiak, Domenico Osella

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

We report on the binding of cisplatin, carboplatin and oxaliplatin to double-stranded DNA in two different (phosphate and carbonate) buffers, using an electrochemical DNA-biosensor. The propensity of the electrophilic agent produced by hydrolysis to interact with DNA was measured as a function of the decrease of guanine oxidation signal of the metal-DNA adduct immobilized on a screen-printed electrode, by using square wave voltammetry. The results obtained confirm that carbonate reacts with platinum drugs to form activated carbonato complexes, which are able to react readily with DNA.

Original languageEnglish (US)
Pages (from-to)1023-1027
Number of pages5
JournalJournal of Inorganic Biochemistry
Volume101
Issue number7
DOIs
StatePublished - Jul 1 2007

Keywords

  • Carbonate buffer
  • DNA-biosensor
  • Platinum drugs

ASJC Scopus subject areas

  • Biochemistry
  • Inorganic Chemistry

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