TCR and Lat are expressed on separate protein islands on T cell membranes and concatenate during activation

Björn F. Lillemeier, Manuel A. Mörtelmaier, Martin B. Forstner, Johannes B. Huppa, Jay T. Groves, Mark M. Davis

Research output: Contribution to journalArticle

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Abstract

The organization and dynamics of receptors and other molecules in the plasma membrane are not well understood. Here we analyzed the spatio-temporal dynamics of T cell antigen receptor (TCR) complexes and linker for activation of T cells (Lat), a key adaptor molecule in the TCR signaling pathway, in T cell membranes using high-speed photoactivated localization microscopy, dual-color fluorescence cross-correlation spectroscopy and transmission electron microscopy. In quiescent T cells, both molecules existed in separate membrane domains (protein islands), and these domains concatenated after T cell activation. These concatemers were identical to signaling microclusters, a prominent hallmark of T cell activation. This separation versus physical juxtapositioning of receptor domains and domains containing downstream signaling molecules in quiescent versus activated T cells may be a general feature of plasma membrane-associated signal transduction.

Original languageEnglish (US)
Pages (from-to)90-96
Number of pages7
JournalNature Immunology
Volume11
Issue number1
DOIs
StatePublished - Jan 2010
Externally publishedYes

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ASJC Scopus subject areas

  • Immunology

Cite this

Lillemeier, B. F., Mörtelmaier, M. A., Forstner, M. B., Huppa, J. B., Groves, J. T., & Davis, M. M. (2010). TCR and Lat are expressed on separate protein islands on T cell membranes and concatenate during activation. Nature Immunology, 11(1), 90-96. https://doi.org/10.1038/ni.1832