TY - JOUR
T1 - Taste receptors and gustatory associated G proteins in channel catfish, Ictalurus punctatus
AU - Gao, Sen
AU - Liu, Shikai
AU - Yao, Jun
AU - Zhou, Tao
AU - Li, Ning
AU - Li, Qi
AU - Dunham, Rex
AU - Liu, Zhanjiang
N1 - Funding Information:
All the bioinformatics analyses in this project were conducted on Alabama Supercomputer Center and the National Science Foundation funded MRI-R2 project #DBI-0959894. This project was supported by USDA National Institute of Food and Agriculture through grants from Agriculture and Food Research Initiative Animal Genomics, Genetics and Breeding Program ( 2015-67015-22907 ) and Animal Disease Program ( 2015-67015-22975 ). S. Gao was supported by a scholarship from the China Scholarship Council (CSC).
Publisher Copyright:
© 2016 Elsevier Inc.
PY - 2017/3/1
Y1 - 2017/3/1
N2 - Taste sensation plays a pivotal role in nutrient identification and acquisition. This is particularly true for channel catfish (Ictalurus punctatus) that live in turbid waters with limited visibility. This biological process is mainly mediated by taste receptors expressed in taste buds that are distributed in several organs and tissues, including the barbels and skin. In the present study, we identified a complete repertoire of taste receptor and gustatory associated G protein genes in the channel catfish genome. A total of eight taste receptor genes were identified, including five type I and three type II taste receptor genes. Their genomic locations, phylogenetic relations, orthologies and expression were determined. Phylogenetic and collinear analyses provided understanding of the evolution dynamics of this gene family. Furthermore, the motif and dN/dS analyses indicated that selection pressures of different degrees were imposed on these receptors. Additionally, four genes of gustatory associated G proteins were also identified. It was indicated that expression patterns of catfish taste receptors and gustatory associated G proteins across organs mirror the distribution of taste buds across organs. Finally, the expression comparison between catfish and zebrafish organs provided evidence of potential roles of catfish skin and gill involved in taste sensation.
AB - Taste sensation plays a pivotal role in nutrient identification and acquisition. This is particularly true for channel catfish (Ictalurus punctatus) that live in turbid waters with limited visibility. This biological process is mainly mediated by taste receptors expressed in taste buds that are distributed in several organs and tissues, including the barbels and skin. In the present study, we identified a complete repertoire of taste receptor and gustatory associated G protein genes in the channel catfish genome. A total of eight taste receptor genes were identified, including five type I and three type II taste receptor genes. Their genomic locations, phylogenetic relations, orthologies and expression were determined. Phylogenetic and collinear analyses provided understanding of the evolution dynamics of this gene family. Furthermore, the motif and dN/dS analyses indicated that selection pressures of different degrees were imposed on these receptors. Additionally, four genes of gustatory associated G proteins were also identified. It was indicated that expression patterns of catfish taste receptors and gustatory associated G proteins across organs mirror the distribution of taste buds across organs. Finally, the expression comparison between catfish and zebrafish organs provided evidence of potential roles of catfish skin and gill involved in taste sensation.
KW - Barbel
KW - Catfish
KW - Evolution
KW - Skin
KW - Taste receptor
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U2 - 10.1016/j.cbd.2016.10.002
DO - 10.1016/j.cbd.2016.10.002
M3 - Article
C2 - 27806254
AN - SCOPUS:84993960711
SN - 1744-117X
VL - 21
SP - 1
EP - 9
JO - Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics
JF - Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics
ER -