T4 phage lysozyme. A protein designed for understanding trypotphan photophysics

Bruce Hudson; Danni Harris

T4 phage lysozyme. A protein designed for understanding trypotphan photophysics

Bruce Hudson, Danni Harris

Research output: Chapter in Book/Entry/Poem › Conference contribution

Abstract

Bacteriophage T4 lysozyme in its wild type form contains three tryptophan residues (at sequence positions 126, 138 and 158). These three residues are in rather different evironments in the proteins: 126 and 158 are near the protein surface while residue 138 is more buried. T4 lysozyme has been genetically engineered to prepare all possible variants in which one or more of the tryptophan residue have been replaced by tyrosine. The available data supports the hypothesis that this substitution has, at most, a very minor effect on the structure of the protein.

Original language	English (US)
Title of host publication	Proceedings of SPIE - The International Society for Optical Engineering
Editors	Joseph F. Lakowicz
Publisher	Publ by Int Soc for Optical Engineering
Pages	80-91
Number of pages	12
ISBN (Print)	0819402451
State	Published - 1990
Externally published	Yes
Event	Time-Resolved Laser Spectroscopy in Biochemistry II - Los Angeles, CA, USA Duration: Jan 15 1990 → Jan 17 1990

Publication series

Name	Proceedings of SPIE - The International Society for Optical Engineering
Volume	1204 pt 1
ISSN (Print)	0277-786X

Other

Other	Time-Resolved Laser Spectroscopy in Biochemistry II
City	Los Angeles, CA, USA
Period	1/15/90 → 1/17/90

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Computer Science Applications
Applied Mathematics
Electrical and Electronic Engineering

Cite this

T4 phage lysozyme. A protein designed for understanding trypotphan photophysics. / Hudson, Bruce; Harris, Danni.
Proceedings of SPIE - The International Society for Optical Engineering. ed. / Joseph F. Lakowicz. Publ by Int Soc for Optical Engineering, 1990. p. 80-91 (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 1204 pt 1).

Research output: Chapter in Book/Entry/Poem › Conference contribution

Hudson, B & Harris, D 1990, T4 phage lysozyme. A protein designed for understanding trypotphan photophysics. in JF Lakowicz (ed.), Proceedings of SPIE - The International Society for Optical Engineering. Proceedings of SPIE - The International Society for Optical Engineering, vol. 1204 pt 1, Publ by Int Soc for Optical Engineering, pp. 80-91, Time-Resolved Laser Spectroscopy in Biochemistry II, Los Angeles, CA, USA, 1/15/90.

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T4 phage lysozyme. A protein designed for understanding trypotphan photophysics

Abstract

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ASJC Scopus subject areas

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