TY - JOUR
T1 - Synchronization of mouse 3T3 and SV40 3T3 cells by way of centrifugal elutriation
AU - Mitchell, B. F.
AU - Tupper, J. T.
N1 - Funding Information:
The authors wish to thank Mr Burt Kenty and Mr Frank Basile of Beckman Instruments for their excellent advice and technical assistance. We also wish to thank Dr Marvin Meistrich for providing us with a pre-print of his work on the mouse-l cell. - This work was supported by grants from the ACS (BC-181) and the NIH (CA-17203-01).
PY - 1977/5
Y1 - 1977/5
N2 - Populations of G1 phase 3T3 and SV40 3T3 mouse fibroblasts have been isolated from exponentially growing cultures by the technique of centrifugal elutriation. Return of the G1 phase cells to growth conditions results in their synchronous passage through the cell cycle, as determined from monitoring of cell number, [3H]thymidine ([3H]TdR) incorporation and fraction of [3H]TdR labeled nuclei. The durations of G1, S and G2 phases are consistent with values obtained by previous investigators using conventional induction techniques for synchronization. The method for isolation of the G1 phase cells is rapid, the yield is high and the process does not appear to alter the temporal aspects of the cell cycle in either cell type.
AB - Populations of G1 phase 3T3 and SV40 3T3 mouse fibroblasts have been isolated from exponentially growing cultures by the technique of centrifugal elutriation. Return of the G1 phase cells to growth conditions results in their synchronous passage through the cell cycle, as determined from monitoring of cell number, [3H]thymidine ([3H]TdR) incorporation and fraction of [3H]TdR labeled nuclei. The durations of G1, S and G2 phases are consistent with values obtained by previous investigators using conventional induction techniques for synchronization. The method for isolation of the G1 phase cells is rapid, the yield is high and the process does not appear to alter the temporal aspects of the cell cycle in either cell type.
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U2 - 10.1016/0014-4827(77)90180-X
DO - 10.1016/0014-4827(77)90180-X
M3 - Article
C2 - 193701
AN - SCOPUS:0017664438
SN - 0014-4827
VL - 106
SP - 351
EP - 355
JO - Experimental Cell Research
JF - Experimental Cell Research
IS - 2
ER -