Synchronization of mouse 3T3 and SV40 3T3 cells by way of centrifugal elutriation

B. F. Mitchell, J. T. Tupper

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

Populations of G1 phase 3T3 and SV40 3T3 mouse fibroblasts have been isolated from exponentially growing cultures by the technique of centrifugal elutriation. Return of the G1 phase cells to growth conditions results in their synchronous passage through the cell cycle, as determined from monitoring of cell number, [3H]thymidine ([3H]TdR) incorporation and fraction of [3H]TdR labeled nuclei. The durations of G1, S and G2 phases are consistent with values obtained by previous investigators using conventional induction techniques for synchronization. The method for isolation of the G1 phase cells is rapid, the yield is high and the process does not appear to alter the temporal aspects of the cell cycle in either cell type.

Original languageEnglish (US)
Pages (from-to)351-355
Number of pages5
JournalExperimental Cell Research
Volume106
Issue number2
DOIs
StatePublished - May 1977

ASJC Scopus subject areas

  • Cell Biology

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