1H-NMR Of U-G-A and U-G-A-A in D2O:. Assignment of nonexchangeable protons and analysis of solution conformation

Michael P. Stone; Philip N. Borer

doi:10.1016/0301-4622(81)85039-9

¹H-NMR Of U-G-A and U-G-A-A in D₂O:. Assignment of nonexchangeable protons and analysis of solution conformation

Michael P. Stone, Philip N. Borer

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

The ribonucleotide oligomers U-G-A and U-G-A-A have been synthesized enzymatically. These oligomers are cognates of the U³³-Gm³⁴-A³⁵-A³⁶ sequence found in the anticodon loop of t-RNA^phe. The ¹H-NMR chemical shifts of the base and ribose HI' protons as well as the couplings. J_1'-2', of the ribose protons have been examined as a function of temperature. Assignments for these resonances have been completed, and used in the analysis of solution conformation for these oligomers. The results are consistent with the A-RNA structure and suggest the absence of alternative ordered solution structures.

Original language	English (US)
Pages (from-to)	363-368
Number of pages	6
Journal	Biophysical Chemistry
Volume	14
Issue number	4
DOIs	https://doi.org/10.1016/0301-4622(81)85039-9
State	Published - Dec 1981

ASJC Scopus subject areas

Biophysics
Biochemistry
Organic Chemistry

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10.1016/0301-4622(81)85039-9

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@article{5997037024b448ee814dfb20e18de9bb,

title = "1H-NMR Of U-G-A and U-G-A-A in D2O:. Assignment of nonexchangeable protons and analysis of solution conformation",

abstract = "The ribonucleotide oligomers U-G-A and U-G-A-A have been synthesized enzymatically. These oligomers are cognates of the U33-Gm34-A35-A36 sequence found in the anticodon loop of t-RNAphe. The 1H-NMR chemical shifts of the base and ribose HI' protons as well as the couplings. J1'-2', of the ribose protons have been examined as a function of temperature. Assignments for these resonances have been completed, and used in the analysis of solution conformation for these oligomers. The results are consistent with the A-RNA structure and suggest the absence of alternative ordered solution structures.",

author = "Stone, {Michael P.} and Borer, {Philip N.}",

note = "Funding Information: This work was supported in part by a grant from the National Institute of Health (GM 24494). Grants from the National Science Foundation (CHE-79-10821 and MPS 75-06152) provided funds for the Bruker WM-250 and WH-90 spectrometers. The 500 MHz spectrometer at the Southern California Regional NMR Facility was supported by the National Science Foundation (CHE-79-1632-Al). We are grateful for assistance to Dr. John Wright at the UC. San Diego NMR Center.",

year = "1981",

month = dec,

doi = "10.1016/0301-4622(81)85039-9",

language = "English (US)",

volume = "14",

pages = "363--368",

journal = "Biophysical Chemistry",

issn = "0301-4622",

publisher = "Elsevier",

number = "4",

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T1 - 1H-NMR Of U-G-A and U-G-A-A in D2O:. Assignment of nonexchangeable protons and analysis of solution conformation

AU - Stone, Michael P.

AU - Borer, Philip N.

N1 - Funding Information: This work was supported in part by a grant from the National Institute of Health (GM 24494). Grants from the National Science Foundation (CHE-79-10821 and MPS 75-06152) provided funds for the Bruker WM-250 and WH-90 spectrometers. The 500 MHz spectrometer at the Southern California Regional NMR Facility was supported by the National Science Foundation (CHE-79-1632-Al). We are grateful for assistance to Dr. John Wright at the UC. San Diego NMR Center.

PY - 1981/12

Y1 - 1981/12

N2 - The ribonucleotide oligomers U-G-A and U-G-A-A have been synthesized enzymatically. These oligomers are cognates of the U33-Gm34-A35-A36 sequence found in the anticodon loop of t-RNAphe. The 1H-NMR chemical shifts of the base and ribose HI' protons as well as the couplings. J1'-2', of the ribose protons have been examined as a function of temperature. Assignments for these resonances have been completed, and used in the analysis of solution conformation for these oligomers. The results are consistent with the A-RNA structure and suggest the absence of alternative ordered solution structures.

AB - The ribonucleotide oligomers U-G-A and U-G-A-A have been synthesized enzymatically. These oligomers are cognates of the U33-Gm34-A35-A36 sequence found in the anticodon loop of t-RNAphe. The 1H-NMR chemical shifts of the base and ribose HI' protons as well as the couplings. J1'-2', of the ribose protons have been examined as a function of temperature. Assignments for these resonances have been completed, and used in the analysis of solution conformation for these oligomers. The results are consistent with the A-RNA structure and suggest the absence of alternative ordered solution structures.

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AN - SCOPUS:0019764580

SN - 0301-4622

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JO - Biophysical Chemistry

JF - Biophysical Chemistry

IS - 4

ER -

¹H-NMR Of U-G-A and U-G-A-A in D₂O:. Assignment of nonexchangeable protons and analysis of solution conformation

Abstract

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