1H-NMR Of U-G-A and U-G-A-A in D2O:. Assignment of nonexchangeable protons and analysis of solution conformation

Michael P. Stone, Philip N. Borer

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

The ribonucleotide oligomers U-G-A and U-G-A-A have been synthesized enzymatically. These oligomers are cognates of the U33-Gm34-A35-A36 sequence found in the anticodon loop of t-RNAphe. The 1H-NMR chemical shifts of the base and ribose HI' protons as well as the couplings. J1'-2', of the ribose protons have been examined as a function of temperature. Assignments for these resonances have been completed, and used in the analysis of solution conformation for these oligomers. The results are consistent with the A-RNA structure and suggest the absence of alternative ordered solution structures.

Original languageEnglish (US)
Pages (from-to)363-368
Number of pages6
JournalBiophysical Chemistry
Volume14
Issue number4
DOIs
StatePublished - Dec 1981

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ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Organic Chemistry

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