The ribonucleotide oligomers U-G-A and U-G-A-A have been synthesized enzymatically. These oligomers are cognates of the U33-Gm34-A35-A36 sequence found in the anticodon loop of t-RNAphe. The 1H-NMR chemical shifts of the base and ribose HI' protons as well as the couplings. J1'-2', of the ribose protons have been examined as a function of temperature. Assignments for these resonances have been completed, and used in the analysis of solution conformation for these oligomers. The results are consistent with the A-RNA structure and suggest the absence of alternative ordered solution structures.
ASJC Scopus subject areas
- Organic Chemistry