1H-NMR of G-U-C and G-U-C-C in D2O:. Assignment of nonexchangeable protons and analysis of solution conformation

Michael P. Stone, Philip N. Borer

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

The ribonucleotide oligomers G-U-C and G-U-C-C have been synthesized enzymatically. These oligomers are cognates of the m7G46-U47-C48-m5C49 sequence found in the variable loop of t-RNAphe. The 1H-NMR chemical shifts of the base and ribose Hl; protons as well as the couplings. J1'-2' of the ribose protons have been examined as a function of temperature. Assignments for these resonances have been completed, and used in the analysis of solution conformation for these oligomers. The results are consistent with the basic features of the A-RNA structure and suggest the absence of alternative ordered solution structures.

Original languageEnglish (US)
Pages (from-to)369-374
Number of pages6
JournalBiophysical Chemistry
Volume14
Issue number4
DOIs
StatePublished - Dec 1981

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Organic Chemistry

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