The ribonucleotide oligomers G-U-C and G-U-C-C have been synthesized enzymatically. These oligomers are cognates of the m7G46-U47-C48-m5C49 sequence found in the variable loop of t-RNAphe. The 1H-NMR chemical shifts of the base and ribose Hl; protons as well as the couplings. J1'-2' of the ribose protons have been examined as a function of temperature. Assignments for these resonances have been completed, and used in the analysis of solution conformation for these oligomers. The results are consistent with the basic features of the A-RNA structure and suggest the absence of alternative ordered solution structures.
ASJC Scopus subject areas
- Organic Chemistry