13C-NMR of ribosyl A-A-A, A-A-G, AND A-U-G Synthesis and Assignment

Michael P. Stone, Stephen A. Winkle, Gloria D. McFarland, Min C. Yoo, Philip N. Borer

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

The three RNA trinucleotides; ApApA, ApApG, and ApUpG, have been synthesized in sufficient quantity to obtain natural abundance 13C{1H}-NMR spectra at strand concentrations between 4 and 100 mM. Comparisons between 70°C spectra of the three trimers and their consistuent dimers ApA, ApG, ApU, and UpG allow secure assignments to be made for most of the resonances. This paper describes the syntheses and 13C assignments of the oligomers.

Original languageEnglish (US)
Pages (from-to)129-138
Number of pages10
JournalBiophysical Chemistry
Volume23
Issue number1-2
DOIs
StatePublished - Nov 1985

Keywords

  • C - NMR
  • Comparative assignment
  • Trinucleotide

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Organic Chemistry

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    Stone, M. P., Winkle, S. A., McFarland, G. D., Yoo, M. C., & Borer, P. N. (1985). 13C-NMR of ribosyl A-A-A, A-A-G, AND A-U-G Synthesis and Assignment. Biophysical Chemistry, 23(1-2), 129-138. https://doi.org/10.1016/0301-4622(85)80071-5