Structure and expression of transferrin gene of channel catfish, Ictalurus punctatus

Hong Liu, Tomokazu Takano, Jason Abernathy, Shaolin Wang, Zhenxia Sha, Yanliang Jiang, Jeffery Terhune, Huseyin Kucuktas, Eric Peatman, Zhanjian "John" Liu

Research output: Contribution to journalArticle

38 Scopus citations

Abstract

Transferrin is important in iron metabolism and has been reported to be involved in disease defence responses after bacterial infection. In this study, we identified, sequenced, and characterized the transferrin gene from channel catfish, Ictalurus punctatus. The catfish transferrin gene was similar to those of other vertebrate species with 17 exons and 16 introns. Sequence analysis indicated the presence of the two duplicated lobes, each containing two sub-domains separated by a cleft harboring the iron-binding site, suggesting their structural conservation. The channel catfish transferrin cDNA encodes 679 amino acids with 42-56% similarity to known transferrin genes from various species. Southern blot analysis suggested the presence of two copies of the transferrin gene in the catfish genome, perhaps arranged in a tandem fashion. The catfish transferrin gene was mapped to a catfish BAC-based physical map. The catfish transferrin gene was highly expressed in the liver, but expression was low in most other tested tissues. Transferrin expression was significantly up-regulated after infection with Edwardsiella ictaluri, the causative agent of enteric septicemia of catfish. Such induction was also found with co-injection of iron-dextran and E. ictaluri, while transferrin expression was not significantly induced with the injection of iron-dextran alone.

Original languageEnglish (US)
Pages (from-to)159-166
Number of pages8
JournalFish and Shellfish Immunology
Volume28
Issue number1
DOIs
StatePublished - Jan 2010
Externally publishedYes

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Keywords

  • Catfish
  • Edwardsiella ictaluri
  • Ictalurus punctatus
  • Infection
  • Iron
  • Teleost
  • Transferrin

ASJC Scopus subject areas

  • Aquatic Science
  • Environmental Chemistry

Cite this

Liu, H., Takano, T., Abernathy, J., Wang, S., Sha, Z., Jiang, Y., Terhune, J., Kucuktas, H., Peatman, E., & Liu, Z. J. (2010). Structure and expression of transferrin gene of channel catfish, Ictalurus punctatus. Fish and Shellfish Immunology, 28(1), 159-166. https://doi.org/10.1016/j.fsi.2009.10.014