Stepwise Assembly and Characterization of DNA Linked Two-Color Quantum Dot Clusters

Kaitlin Coopersmith, Hyunjoo Han, Mathew M Maye

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The DNA-mediated self-assembly of multicolor quantum dot (QD) clusters via a stepwise approach is described. The CdSe/ZnS QDs were synthesized and functionalized with an amphiphilic copolymer, followed by ssDNA conjugation. At each functionalization step, the QDs were purified via gradient ultracentrifugation, which was found to remove excess polymer and QD aggregates, allowing for improved conjugation yields and assembly reactivity. The QDs were then assembled and disassembled in a stepwise manner at a ssDNA functionalized magnetic colloid, which provided a convenient way to remove unreacted QDs and ssDNA impurities. After assembly/disassembly, the clusters' optical characteristics were studied by fluorescence spectroscopy and the assembly morphology and stoichiometry was imaged via electron microscopy. The results indicate that a significant amount of QD-to-QD energy transfer occurred in the clusters, which was studied as a function of increasing acceptor-to-donor ratios, resulting in increased QD acceptor emission intensities compared to controls.

Original languageEnglish (US)
Pages (from-to)7463-7471
Number of pages9
JournalLangmuir
Volume31
Issue number27
DOIs
StatePublished - Jul 1 2015

Fingerprint

Semiconductor quantum dots
DNA
deoxyribonucleic acid
assembly
quantum dots
Color
color
conjugation
Fluorescence spectroscopy
Colloids
Stoichiometry
Energy transfer
Self assembly
Electron microscopy
colloids
self assembly
stoichiometry
electron microscopy
copolymers
Polymers

ASJC Scopus subject areas

  • Electrochemistry
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Materials Science(all)
  • Spectroscopy

Cite this

Stepwise Assembly and Characterization of DNA Linked Two-Color Quantum Dot Clusters. / Coopersmith, Kaitlin; Han, Hyunjoo; Maye, Mathew M.

In: Langmuir, Vol. 31, No. 27, 01.07.2015, p. 7463-7471.

Research output: Contribution to journalArticle

Coopersmith, Kaitlin ; Han, Hyunjoo ; Maye, Mathew M. / Stepwise Assembly and Characterization of DNA Linked Two-Color Quantum Dot Clusters. In: Langmuir. 2015 ; Vol. 31, No. 27. pp. 7463-7471.
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