Stability of ribonucleic acid double-stranded helices

Philip N. Borer, Barbara Dengler, Ignacio Tinoco, Olke C. Uhlenbeck

Research output: Contribution to journalArticle

590 Citations (Scopus)

Abstract

The hypochromicity, as a function of temperature for 19 oligoribonucleotides capable of forming perfectly base-paired double helices, is used to extract thermodynamic parameters of helix formation. The data are analyzed by an all or none model of helix melting which permits assignment of ΔG, ΔH, and ΔS of formation to each of the ten possible Watson-Crick base-paired nearest-neighbor sequences. Helix stability is found to have a striking dependence on sequence, and formulae are provided to predict the Tm of any RNA double helix of known sequence.

Original languageEnglish (US)
Pages (from-to)843-853
Number of pages11
JournalJournal of Molecular Biology
Volume86
Issue number4
DOIs
StatePublished - Jul 15 1974

Fingerprint

Oligoribonucleotides
Thermodynamics
Freezing
RNA
Temperature

ASJC Scopus subject areas

  • Virology

Cite this

Borer, P. N., Dengler, B., Tinoco, I., & Uhlenbeck, O. C. (1974). Stability of ribonucleic acid double-stranded helices. Journal of Molecular Biology, 86(4), 843-853. https://doi.org/10.1016/0022-2836(74)90357-X

Stability of ribonucleic acid double-stranded helices. / Borer, Philip N.; Dengler, Barbara; Tinoco, Ignacio; Uhlenbeck, Olke C.

In: Journal of Molecular Biology, Vol. 86, No. 4, 15.07.1974, p. 843-853.

Research output: Contribution to journalArticle

Borer, PN, Dengler, B, Tinoco, I & Uhlenbeck, OC 1974, 'Stability of ribonucleic acid double-stranded helices', Journal of Molecular Biology, vol. 86, no. 4, pp. 843-853. https://doi.org/10.1016/0022-2836(74)90357-X
Borer, Philip N. ; Dengler, Barbara ; Tinoco, Ignacio ; Uhlenbeck, Olke C. / Stability of ribonucleic acid double-stranded helices. In: Journal of Molecular Biology. 1974 ; Vol. 86, No. 4. pp. 843-853.
@article{553afc6573454785aef3580956f10a85,
title = "Stability of ribonucleic acid double-stranded helices",
abstract = "The hypochromicity, as a function of temperature for 19 oligoribonucleotides capable of forming perfectly base-paired double helices, is used to extract thermodynamic parameters of helix formation. The data are analyzed by an all or none model of helix melting which permits assignment of ΔG, ΔH, and ΔS of formation to each of the ten possible Watson-Crick base-paired nearest-neighbor sequences. Helix stability is found to have a striking dependence on sequence, and formulae are provided to predict the Tm of any RNA double helix of known sequence.",
author = "Borer, {Philip N.} and Barbara Dengler and Ignacio Tinoco and Uhlenbeck, {Olke C.}",
year = "1974",
month = "7",
day = "15",
doi = "10.1016/0022-2836(74)90357-X",
language = "English (US)",
volume = "86",
pages = "843--853",
journal = "Journal of Molecular Biology",
issn = "0022-2836",
publisher = "Academic Press Inc.",
number = "4",

}

TY - JOUR

T1 - Stability of ribonucleic acid double-stranded helices

AU - Borer, Philip N.

AU - Dengler, Barbara

AU - Tinoco, Ignacio

AU - Uhlenbeck, Olke C.

PY - 1974/7/15

Y1 - 1974/7/15

N2 - The hypochromicity, as a function of temperature for 19 oligoribonucleotides capable of forming perfectly base-paired double helices, is used to extract thermodynamic parameters of helix formation. The data are analyzed by an all or none model of helix melting which permits assignment of ΔG, ΔH, and ΔS of formation to each of the ten possible Watson-Crick base-paired nearest-neighbor sequences. Helix stability is found to have a striking dependence on sequence, and formulae are provided to predict the Tm of any RNA double helix of known sequence.

AB - The hypochromicity, as a function of temperature for 19 oligoribonucleotides capable of forming perfectly base-paired double helices, is used to extract thermodynamic parameters of helix formation. The data are analyzed by an all or none model of helix melting which permits assignment of ΔG, ΔH, and ΔS of formation to each of the ten possible Watson-Crick base-paired nearest-neighbor sequences. Helix stability is found to have a striking dependence on sequence, and formulae are provided to predict the Tm of any RNA double helix of known sequence.

UR - http://www.scopus.com/inward/record.url?scp=0016189342&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0016189342&partnerID=8YFLogxK

U2 - 10.1016/0022-2836(74)90357-X

DO - 10.1016/0022-2836(74)90357-X

M3 - Article

C2 - 4427357

AN - SCOPUS:0016189342

VL - 86

SP - 843

EP - 853

JO - Journal of Molecular Biology

JF - Journal of Molecular Biology

SN - 0022-2836

IS - 4

ER -