Stability of ribonucleic acid double-stranded helices

Philip N. Borer, Barbara Dengler, Ignacio Tinoco, Olke C. Uhlenbeck

Research output: Contribution to journalArticlepeer-review

633 Scopus citations


The hypochromicity, as a function of temperature for 19 oligoribonucleotides capable of forming perfectly base-paired double helices, is used to extract thermodynamic parameters of helix formation. The data are analyzed by an all or none model of helix melting which permits assignment of ΔG, ΔH, and ΔS of formation to each of the ten possible Watson-Crick base-paired nearest-neighbor sequences. Helix stability is found to have a striking dependence on sequence, and formulae are provided to predict the Tm of any RNA double helix of known sequence.

Original languageEnglish (US)
Pages (from-to)843-853
Number of pages11
JournalJournal of Molecular Biology
Issue number4
StatePublished - Jul 15 1974

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology


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