Stability of ribonucleic acid double-stranded helices

Philip N. Borer; Barbara Dengler; Ignacio Tinoco; Olke C. Uhlenbeck

doi:10.1016/0022-2836(74)90357-X

Stability of ribonucleic acid double-stranded helices

Philip N. Borer, Barbara Dengler, Ignacio Tinoco, Olke C. Uhlenbeck

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

635 Scopus citations

Abstract

The hypochromicity, as a function of temperature for 19 oligoribonucleotides capable of forming perfectly base-paired double helices, is used to extract thermodynamic parameters of helix formation. The data are analyzed by an all or none model of helix melting which permits assignment of ΔG, ΔH, and ΔS of formation to each of the ten possible Watson-Crick base-paired nearest-neighbor sequences. Helix stability is found to have a striking dependence on sequence, and formulae are provided to predict the T_m of any RNA double helix of known sequence.

Original language	English (US)
Pages (from-to)	843-853
Number of pages	11
Journal	Journal of Molecular Biology
Volume	86
Issue number	4
DOIs	https://doi.org/10.1016/0022-2836(74)90357-X
State	Published - Jul 15 1974

ASJC Scopus subject areas

Structural Biology
Molecular Biology

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10.1016/0022-2836(74)90357-X

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title = "Stability of ribonucleic acid double-stranded helices",

abstract = "The hypochromicity, as a function of temperature for 19 oligoribonucleotides capable of forming perfectly base-paired double helices, is used to extract thermodynamic parameters of helix formation. The data are analyzed by an all or none model of helix melting which permits assignment of ΔG, ΔH, and ΔS of formation to each of the ten possible Watson-Crick base-paired nearest-neighbor sequences. Helix stability is found to have a striking dependence on sequence, and formulae are provided to predict the Tm of any RNA double helix of known sequence.",

author = "Borer, {Philip N.} and Barbara Dengler and Ignacio Tinoco and Uhlenbeck, {Olke C.}",

note = "Funding Information: This work was supported by grants from the National Institutes of Health; GM10840 to one of us (I. T.), GM41393 to another author (P. N. B.) and GM19059 to a third author (0. C. U.). We thank Mr Cemal Kemal and Mr David Koh for their excellent technical assistance. One of us (0. C. U.) is a recipient of a Career Development Award from the National Institutes of Health.",

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doi = "10.1016/0022-2836(74)90357-X",

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T1 - Stability of ribonucleic acid double-stranded helices

AU - Borer, Philip N.

AU - Dengler, Barbara

AU - Tinoco, Ignacio

AU - Uhlenbeck, Olke C.

N1 - Funding Information: This work was supported by grants from the National Institutes of Health; GM10840 to one of us (I. T.), GM41393 to another author (P. N. B.) and GM19059 to a third author (0. C. U.). We thank Mr Cemal Kemal and Mr David Koh for their excellent technical assistance. One of us (0. C. U.) is a recipient of a Career Development Award from the National Institutes of Health.

PY - 1974/7/15

Y1 - 1974/7/15

N2 - The hypochromicity, as a function of temperature for 19 oligoribonucleotides capable of forming perfectly base-paired double helices, is used to extract thermodynamic parameters of helix formation. The data are analyzed by an all or none model of helix melting which permits assignment of ΔG, ΔH, and ΔS of formation to each of the ten possible Watson-Crick base-paired nearest-neighbor sequences. Helix stability is found to have a striking dependence on sequence, and formulae are provided to predict the Tm of any RNA double helix of known sequence.

AB - The hypochromicity, as a function of temperature for 19 oligoribonucleotides capable of forming perfectly base-paired double helices, is used to extract thermodynamic parameters of helix formation. The data are analyzed by an all or none model of helix melting which permits assignment of ΔG, ΔH, and ΔS of formation to each of the ten possible Watson-Crick base-paired nearest-neighbor sequences. Helix stability is found to have a striking dependence on sequence, and formulae are provided to predict the Tm of any RNA double helix of known sequence.

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JF - Journal of Molecular Biology

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Stability of ribonucleic acid double-stranded helices

Abstract

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