Spreading rates of bacterial colonies depend on substrate stiffness and permeability

Merrill E. Asp, Minh Tri Ho Thanh, Danielle A. Germann, Robert J. Carroll, Alana Franceski, Roy D. Welch, Arvind Gopinath, Alison E. Patteson

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

The ability of bacteria to colonize and grow on different surfaces is an essential process for biofilm development. Here, we report the use of synthetic hydrogels with tunable stiffness and porosity to assess physical effects of the substrate on biofilm development. Using time-lapse microscopy to track the growth of expanding Serratia marcescens colonies, we find that biofilm colony growth can increase with increasing substrate stiffness, unlike what is found on traditional agar substrates. Using traction force microscopy-based techniques, we find that biofilms exert transient stresses correlated over length scales much larger than a single bacterium, and that the magnitude of these forces also increases with increasing substrate stiffness. Our results are consistent with a model of biofilm development in which the interplay between osmotic pressure arising from the biofilm and the poroelastic response of the underlying substrate controls biofilm growth and morphology.

Original languageEnglish (US)
Article numberpgac025
JournalPNAS Nexus
Volume1
Issue number1
DOIs
StatePublished - Mar 1 2022

Keywords

  • bacterial colonies
  • biofilms
  • hydrogels
  • mechanobiology
  • traction force microscopy

ASJC Scopus subject areas

  • General

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