Abstract
Lambert-Eaton Myasthenic Syndrome (LEMS) is a presynaptic, neuromuscular disorder characterized by impaired nerve-evoked release of ACh. Repetitive nerve stimulation, which increases the probability of quantal release, improves the transmission defect. An autoantibody to Ca2+ channels of presynaptic motor nerve terminals is thought to mediate the pathogenesis of this disease. The goal of the present study was to examine the specificity of LEMS autoantibodies for nerve terminal Ca2+ channels as compared to other voltage-sensitive ion channels in nerve terminals, and to determine if non-specific membrane damage contributed to the pathogenesis of LEMS. The ion channel specificity of LEMS autoantibody was assessed by comparing the ability of acute application of IgG isolated from the plasma of a patient with LEMS to reduce depolarization-dependent uptake of 45Ca2+ and 22Na+ into or efflux of 86Rb+ from rat forebrain synaptosomes. The clinical diagnosis of LEMS was confirmed electrophysiologically by treatment of mice for 30 days with plasma (1.5 ml/day) taken from this patient. Characteristic reduction of quantal content elicited at 1 Hz and facilitation at 20 Hz was observed in mice treated wtih LEMS plasma compared to those treated with control plasma. One s, K+-stimulated 45Ca2+ uptake was inhibited 36.5 ± 14.5% and 44.5 ± 9.8% by acute application of 2 and 4 mg/ml LEMS IgG, respectively; IgG from patients with small cell carcinoma of the lung (SCC) had no effect on 45Ca2+ entry. The same concentrations of LEMS IgG affected neither voltage-dependent uptake of 22Na+ into veratridine-depolarized synaptosomes nor 86R+ efflux from K+-depolarized synaptosomes. Lactate dehydrogenase release from synaptosomes incubated with varying concentrations of LEMS IgG was not increased over control suggesting that the reduction in 45Ca2+ uptake was not a result of disruption of synaptosomal membrane integrity. Likewise, normal activation of Ca2+ channels was not prevented by IgG-induced alterations in synaptosomal membrane potentials as the fluorescence of a membrane potential-sensitive carbocyanine dye (diS-C2(5)) was unaltered following acute application of 2 mg/ml LEMS or control IgG. These results indicate that immunoglobulins isolated from a patient with LEMS alter presynaptic nerve terminals Ca2+ channel activity. These deficits are not due to decreases in synaptosomal integrity nor to IgG-induced alterations in the synaptosomal membrane potential but appear to result from direct action on voltage-dependent Ca2+ channels. The antibody from this patient appears to be specific for nerve terminal Ca2+ channels as it did not affect ion flux through voltage-dependent Na+ or K+ channels.
Original language | English (US) |
---|---|
Pages (from-to) | 324-332 |
Number of pages | 9 |
Journal | Brain Research |
Volume | 599 |
Issue number | 2 |
DOIs | |
State | Published - Dec 25 1992 |
Externally published | Yes |
Keywords
- Autoantibody
- Calcium channel
- Isolated nerve terminal (synaptosome)
- Neuromuscular disease
- Potassium channel
- Sodium channel
ASJC Scopus subject areas
- General Neuroscience
- Molecular Biology
- Clinical Neurology
- Developmental Biology