SNP Discovery through EST Data Mining

Shaolin Wang, Zhanjiang John Liu

Research output: Chapter in Book/Entry/PoemChapter

1 Scopus citations
Original languageEnglish (US)
Title of host publicationNext Generation Sequencing and Whole Genome Selection in Aquaculture
PublisherWiley Blackwell
Pages91-108
Number of pages18
ISBN (Print)9780813806372
DOIs
StatePublished - Dec 10 2010
Externally publishedYes

Keywords

  • CLC Genomics Workbench, package - integrating analysis functions for nucleotide and protein sequences, as sequence assembly
  • EST-derived SNPs - correlating genes in terms of genomic locations
  • Identification of SNP using ClustalW - and NCBI BLAST
  • Key issue of single-nucleotide polymorphism (SNP) marker applications - in aquaculture species, availability of SNPs
  • Multiple alignment tool, The ClustalX/ClustalW program - for protein and nucleic acid multiple sequence alignments and phylogenetic tree construction
  • SNP discovery through EST data mining
  • SNP discovery through EST data mining - advantages and disadvantages
  • SNP discovery using EST, alignment of multiple EST sequences - from a single transcript
  • SNP discovery using ESTs - generated by Sanger sequencing
  • Transcript sequences, using next generation sequencing platforms - as Illumina Genome Analyzer and Roche 454 sequencer

ASJC Scopus subject areas

  • General Agricultural and Biological Sciences

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