Small RNA library cloning procedure for deep sequencing of specific endogenous siRNA classes in Caenorhabditis elegans

Maria C. Ow, Nelson C. Lau, Sarah E. Hall

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

In recent years, distinct classes of small RNAs ranging in size from ~21 to 26 nucleotides have been discovered and shown to play important roles in a wide array of cellular functions. Because of the abundance of these small RNAs, library preparation from an RNA sample followed by deep sequencing provides the identity and quantity of a particular class of small RNAs. In this chapter we describe a detailed protocol for preparing small RNA libraries for deep sequencing on the Illumina platform from the nematode C. elegans.

Original languageEnglish (US)
Pages (from-to)59-70
Number of pages12
JournalMethods in Molecular Biology
Volume1173
DOIs
StatePublished - 2014

Keywords

  • 22G-siRNA
  • 26G-siRNA
  • C elegans
  • Endogenous siRNAs
  • RNAi
  • Small RNA library cloning

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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