Reaction of vitamin B12 (B12) with excess 2-iodoxybenzoic acid and 2-hydroxypyridine leads to selective oxidation of the 5'-hydroxyl group of the ribose tail of B12 in a 30% isolated yield. The acid derivative was purified in one step by HPLC chromatography and characterized by MALDI-TOF mass spectrometry, 1H NMR and 2D (HSQC and HMBC) NMR. The new carboxylic acid derivative is perfectly suited to make stable amide-based B12 bioconjugates.
|Original language||English (US)|
|Number of pages||4|
|State||Published - Dec 1 2012|
- 2-iodoxybenzoic acid
- Carboxylic acids
ASJC Scopus subject areas
- Organic Chemistry