Site-selective binding of nanoparticles to double-stranded DNA via peptide nucleic acid "invasion"

Andrea L. Stadler, Dazhi Sun, Mathew M. Maye, Daniel Van Der Lelie, Oleg Gang

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

We demonstrate a novel method for by-design placement of nano-objects along double-stranded (ds) DNA. A molecular intercalator, designed as a peptide nucleic acid (PNA)-DNA chimera, is able to invade dsDNA at the PNA-side due to the hybridization specificity between PNA and one of the duplex strands. At the same time, the single-stranded (ss) DNA tail of the chimera, allows for anchoring of nano-objects that have been functionalized with complementary ssDNA. The developed method is applied for interparticle attachment and for the fabrication of particle clusters using a dsDNA template. This method significantly broadens the molecular toolbox for constructing nanoscale systems by including the most conventional not yet utilized DNA motif, double helix DNA.

Original languageEnglish (US)
Pages (from-to)2467-2474
Number of pages8
JournalACS nano
Volume5
Issue number4
DOIs
StatePublished - Apr 26 2011

Keywords

  • double-stranded DNA
  • gold nanoparticles
  • nanoparticle assembly
  • peptide nucleic acid

ASJC Scopus subject areas

  • Materials Science(all)
  • Engineering(all)
  • Physics and Astronomy(all)

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