Site-selective binding of nanoparticles to double-stranded DNA via peptide nucleic acid "invasion"

Andrea L. Stadler; Dazhi Sun; Mathew M. Maye; Daniel Van Der Lelie; Oleg Gang

doi:10.1021/nn101355n

Site-selective binding of nanoparticles to double-stranded DNA via peptide nucleic acid "invasion"

Andrea L. Stadler, Dazhi Sun, Mathew M. Maye, Daniel Van Der Lelie, Oleg Gang

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

We demonstrate a novel method for by-design placement of nano-objects along double-stranded (ds) DNA. A molecular intercalator, designed as a peptide nucleic acid (PNA)-DNA chimera, is able to invade dsDNA at the PNA-side due to the hybridization specificity between PNA and one of the duplex strands. At the same time, the single-stranded (ss) DNA tail of the chimera, allows for anchoring of nano-objects that have been functionalized with complementary ssDNA. The developed method is applied for interparticle attachment and for the fabrication of particle clusters using a dsDNA template. This method significantly broadens the molecular toolbox for constructing nanoscale systems by including the most conventional not yet utilized DNA motif, double helix DNA.

Original language	English (US)
Pages (from-to)	2467-2474
Number of pages	8
Journal	ACS nano
Volume	5
Issue number	4
DOIs	https://doi.org/10.1021/nn101355n
State	Published - Apr 26 2011

Keywords

double-stranded DNA
gold nanoparticles
nanoparticle assembly
peptide nucleic acid

ASJC Scopus subject areas

General Materials Science
General Engineering
General Physics and Astronomy

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10.1021/nn101355n

Cite this

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abstract = "We demonstrate a novel method for by-design placement of nano-objects along double-stranded (ds) DNA. A molecular intercalator, designed as a peptide nucleic acid (PNA)-DNA chimera, is able to invade dsDNA at the PNA-side due to the hybridization specificity between PNA and one of the duplex strands. At the same time, the single-stranded (ss) DNA tail of the chimera, allows for anchoring of nano-objects that have been functionalized with complementary ssDNA. The developed method is applied for interparticle attachment and for the fabrication of particle clusters using a dsDNA template. This method significantly broadens the molecular toolbox for constructing nanoscale systems by including the most conventional not yet utilized DNA motif, double helix DNA.",

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AU - Stadler, Andrea L.

AU - Sun, Dazhi

AU - Maye, Mathew M.

AU - Van Der Lelie, Daniel

AU - Gang, Oleg

PY - 2011/4/26

Y1 - 2011/4/26

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AB - We demonstrate a novel method for by-design placement of nano-objects along double-stranded (ds) DNA. A molecular intercalator, designed as a peptide nucleic acid (PNA)-DNA chimera, is able to invade dsDNA at the PNA-side due to the hybridization specificity between PNA and one of the duplex strands. At the same time, the single-stranded (ss) DNA tail of the chimera, allows for anchoring of nano-objects that have been functionalized with complementary ssDNA. The developed method is applied for interparticle attachment and for the fabrication of particle clusters using a dsDNA template. This method significantly broadens the molecular toolbox for constructing nanoscale systems by including the most conventional not yet utilized DNA motif, double helix DNA.

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Site-selective binding of nanoparticles to double-stranded DNA via peptide nucleic acid "invasion"

Abstract

Keywords

ASJC Scopus subject areas

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