Single-Molecule Pore-Based Sensor for Proteins and Transient Protein-Protein Interactions

Avinash Kumar Thakur (Inventor), Liviu Movileanu (Inventor)

Research output: Patent


A bioinspired protein pore-based nanostructure that can provide selective, real-time sampling of protein-protein interactions at single-molecule resolution. This modular nanostructure relied on a single polypeptide chain that encompassed a heavily truncated outer membrane protein, a highly flexible connector, a protein receptor element, as well as a polypeptide adapter. The presence of a protein ligand analyte in solution produced reversible binding and release events, in the form of discrete and stochastic current transitions between open substates of the transmembrane pore, the nature of which depend on both the amount of protein ligand analyte and the strength of the transient PPIs in aqueous phase.
Original languageEnglish (US)
Patent number10,921,309
Priority date11/1/17
Filing date11/1/18
StateAccepted/In press - May 2 2019


Dive into the research topics of 'Single-Molecule Pore-Based Sensor for Proteins and Transient Protein-Protein Interactions'. Together they form a unique fingerprint.

Cite this