Single-molecule detection of proteins using nanopores

Research output: ResearchChapter

  • 3 Citations

Abstract

Recently, protein and synthetic nanopores have been employed extensively as single-molecule probes to illuminate the functional features of proteins, including their binding affinity to different ligands, backbone flexibility, enzymatic activity and folding state. In this chapter, I present a brief overview in this emerging area of biosensing. The underlying principle of detection is that the device is based upon a single nanopore drilled into an insulating membrane, which is immersed in a symmetric chamber containing electrolyte solution. The application of a transmembrane potential across the membrane will enable the recording of a well-defined electric current due to the flow of ions crossing the nanopore. The partitioning of single proteins into the interior of the nanopore is detected by discrete current fluctuations that depend upon the interaction between the proteins and the nanopore. The detection mechanisms include chemical modification and genetic engineering of protein nanopores, electrophoretic capture of proteins via movable nucleic acid arms, and functionalization of the inner surface of synthetic nanopores. This approach holds promise for the exploration of proteins at high temporal and spatial resolution. Moreover, nanopore probe techniques can be employed in high-throughput devices used in biomedical molecular diagnosis and environmental monitoring.

LanguageEnglish (US)
Title of host publicationFrontiers in Sensing: From Biology to Engineering
PublisherSpringer-Verlag Wien
Pages363-381
Number of pages19
ISBN (Print)9783211997499, 3211997482, 9783211997482
DOIs
StatePublished - Nov 1 2013

Fingerprint

Nanopores
Proteins
proteins
nanopores
Equipment and Supplies
Membranes
Chemical Engineering
Protein Engineering
Environmental Monitoring
Membrane Potentials
Nucleic Acids
Electrolytes
Ions
Ligands
protein engineering
electric current
environmental monitoring
membrane potential
nucleic acids
electrolytes

ASJC Scopus subject areas

  • Neuroscience(all)
  • Agricultural and Biological Sciences(all)
  • Medicine(all)

Cite this

Movileanu, L. (2013). Single-molecule detection of proteins using nanopores. In Frontiers in Sensing: From Biology to Engineering (pp. 363-381). Springer-Verlag Wien. DOI: 10.1007/978-3-211-99749-9_25

Single-molecule detection of proteins using nanopores. / Movileanu, Liviu.

Frontiers in Sensing: From Biology to Engineering. Springer-Verlag Wien, 2013. p. 363-381.

Research output: ResearchChapter

Movileanu, L 2013, Single-molecule detection of proteins using nanopores. in Frontiers in Sensing: From Biology to Engineering. Springer-Verlag Wien, pp. 363-381. DOI: 10.1007/978-3-211-99749-9_25
Movileanu L. Single-molecule detection of proteins using nanopores. In Frontiers in Sensing: From Biology to Engineering. Springer-Verlag Wien. 2013. p. 363-381. Available from, DOI: 10.1007/978-3-211-99749-9_25
Movileanu, Liviu. / Single-molecule detection of proteins using nanopores. Frontiers in Sensing: From Biology to Engineering. Springer-Verlag Wien, 2013. pp. 363-381
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