Shell microelectrode arrays (MEAs) for brain organoids

Qi Huang, Bohao Tang, July Carolina Romero, Yuqian Yang, Saifeldeen Khalil Elsayed, Gayatri Pahapale, Tien Jung Lee, Itzy E.Morales Pantoja, Fang Han, Cynthia Berlinicke, Terry Xiang, Mallory Solazzo, Thomas Hartung, Zhao Qin, Brian S. Caffo, Lena Smirnova, David H. Gracias

Research output: Contribution to journalArticlepeer-review

36 Scopus citations


Brain organoids are important models for mimicking some three-dimensional (3D) cytoarchitectural and functional aspects of the brain. Multielectrode arrays (MEAs) that enable recording and stimulation of activity from electrogenic cells offer notable potential for interrogating brain organoids. However, conventional MEAs, initially designed for monolayer cultures, offer limited recording contact area restricted to the bottom of the 3D organoids. Inspired by the shape of electroencephalography caps, we developed miniaturized wafer-integrated MEA caps for organoids. The optically transparent shells are composed of self-folding polymer leaflets with conductive polymer-coated metal electrodes. Tunable folding of the minicaps' polymer leaflets guided by mechanics simulations enables versatile recording from organoids of different sizes, and we validate the feasibility of electrophysiology recording from 400- to 600-μm-sized organoids for up to 4 weeks and in response to glutamate stimulation. Our studies suggest that 3D shell MEAs offer great potential for high signal-to-noise ratio and 3D spatiotemporal brain organoid recording.

Original languageEnglish (US)
Article numbereabq5031
JournalScience Advances
Issue number33
StatePublished - Aug 2022
Externally publishedYes

ASJC Scopus subject areas

  • General


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