Shell microelectrode arrays (MEAs) for brain organoids

Qi Huang; Bohao Tang; July Carolina Romero; Yuqian Yang; Saifeldeen Khalil Elsayed; Gayatri Pahapale; Tien Jung Lee; Itzy E.Morales Pantoja; Fang Han; Cynthia Berlinicke; Terry Xiang; Mallory Solazzo; Thomas Hartung; Zhao Qin; Brian S. Caffo; Lena Smirnova; David H. Gracias

doi:10.1126/sciadv.abq5031

Shell microelectrode arrays (MEAs) for brain organoids

Qi Huang, Bohao Tang, July Carolina Romero, Yuqian Yang, Saifeldeen Khalil Elsayed, Gayatri Pahapale, Tien Jung Lee, Itzy E.Morales Pantoja, Fang Han, Cynthia Berlinicke, Terry Xiang, Mallory Solazzo, Thomas Hartung, Zhao Qin, Brian S. Caffo, Lena Smirnova, David H. Gracias

Research output: Contribution to journal › Article › peer-review

72 Scopus citations

Abstract

Brain organoids are important models for mimicking some three-dimensional (3D) cytoarchitectural and functional aspects of the brain. Multielectrode arrays (MEAs) that enable recording and stimulation of activity from electrogenic cells offer notable potential for interrogating brain organoids. However, conventional MEAs, initially designed for monolayer cultures, offer limited recording contact area restricted to the bottom of the 3D organoids. Inspired by the shape of electroencephalography caps, we developed miniaturized wafer-integrated MEA caps for organoids. The optically transparent shells are composed of self-folding polymer leaflets with conductive polymer-coated metal electrodes. Tunable folding of the minicaps' polymer leaflets guided by mechanics simulations enables versatile recording from organoids of different sizes, and we validate the feasibility of electrophysiology recording from 400- to 600-μm-sized organoids for up to 4 weeks and in response to glutamate stimulation. Our studies suggest that 3D shell MEAs offer great potential for high signal-to-noise ratio and 3D spatiotemporal brain organoid recording.

Original language	English (US)
Article number	eabq5031
Journal	Science Advances
Volume	8
Issue number	33
DOIs	https://doi.org/10.1126/sciadv.abq5031
State	Published - Aug 2022
Externally published	Yes

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title = "Shell microelectrode arrays (MEAs) for brain organoids",

abstract = "Brain organoids are important models for mimicking some three-dimensional (3D) cytoarchitectural and functional aspects of the brain. Multielectrode arrays (MEAs) that enable recording and stimulation of activity from electrogenic cells offer notable potential for interrogating brain organoids. However, conventional MEAs, initially designed for monolayer cultures, offer limited recording contact area restricted to the bottom of the 3D organoids. Inspired by the shape of electroencephalography caps, we developed miniaturized wafer-integrated MEA caps for organoids. The optically transparent shells are composed of self-folding polymer leaflets with conductive polymer-coated metal electrodes. Tunable folding of the minicaps' polymer leaflets guided by mechanics simulations enables versatile recording from organoids of different sizes, and we validate the feasibility of electrophysiology recording from 400- to 600-μm-sized organoids for up to 4 weeks and in response to glutamate stimulation. Our studies suggest that 3D shell MEAs offer great potential for high signal-to-noise ratio and 3D spatiotemporal brain organoid recording.",

author = "Qi Huang and Bohao Tang and Romero, {July Carolina} and Yuqian Yang and Elsayed, {Saifeldeen Khalil} and Gayatri Pahapale and Lee, {Tien Jung} and Pantoja, {Itzy E.Morales} and Fang Han and Cynthia Berlinicke and Terry Xiang and Mallory Solazzo and Thomas Hartung and Zhao Qin and Caffo, {Brian S.} and Lena Smirnova and Gracias, {David H.}",

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year = "2022",

month = aug,

doi = "10.1126/sciadv.abq5031",

language = "English (US)",

volume = "8",

journal = "Science Advances",

issn = "2375-2548",

publisher = "American Association for the Advancement of Science",

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T1 - Shell microelectrode arrays (MEAs) for brain organoids

AU - Huang, Qi

AU - Tang, Bohao

AU - Romero, July Carolina

AU - Yang, Yuqian

AU - Elsayed, Saifeldeen Khalil

AU - Pahapale, Gayatri

AU - Lee, Tien Jung

AU - Pantoja, Itzy E.Morales

AU - Han, Fang

AU - Berlinicke, Cynthia

AU - Xiang, Terry

AU - Solazzo, Mallory

AU - Hartung, Thomas

AU - Qin, Zhao

AU - Caffo, Brian S.

AU - Smirnova, Lena

AU - Gracias, David H.

PY - 2022/8

Y1 - 2022/8

N2 - Brain organoids are important models for mimicking some three-dimensional (3D) cytoarchitectural and functional aspects of the brain. Multielectrode arrays (MEAs) that enable recording and stimulation of activity from electrogenic cells offer notable potential for interrogating brain organoids. However, conventional MEAs, initially designed for monolayer cultures, offer limited recording contact area restricted to the bottom of the 3D organoids. Inspired by the shape of electroencephalography caps, we developed miniaturized wafer-integrated MEA caps for organoids. The optically transparent shells are composed of self-folding polymer leaflets with conductive polymer-coated metal electrodes. Tunable folding of the minicaps' polymer leaflets guided by mechanics simulations enables versatile recording from organoids of different sizes, and we validate the feasibility of electrophysiology recording from 400- to 600-μm-sized organoids for up to 4 weeks and in response to glutamate stimulation. Our studies suggest that 3D shell MEAs offer great potential for high signal-to-noise ratio and 3D spatiotemporal brain organoid recording.

AB - Brain organoids are important models for mimicking some three-dimensional (3D) cytoarchitectural and functional aspects of the brain. Multielectrode arrays (MEAs) that enable recording and stimulation of activity from electrogenic cells offer notable potential for interrogating brain organoids. However, conventional MEAs, initially designed for monolayer cultures, offer limited recording contact area restricted to the bottom of the 3D organoids. Inspired by the shape of electroencephalography caps, we developed miniaturized wafer-integrated MEA caps for organoids. The optically transparent shells are composed of self-folding polymer leaflets with conductive polymer-coated metal electrodes. Tunable folding of the minicaps' polymer leaflets guided by mechanics simulations enables versatile recording from organoids of different sizes, and we validate the feasibility of electrophysiology recording from 400- to 600-μm-sized organoids for up to 4 weeks and in response to glutamate stimulation. Our studies suggest that 3D shell MEAs offer great potential for high signal-to-noise ratio and 3D spatiotemporal brain organoid recording.

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Shell microelectrode arrays (MEAs) for brain organoids

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