Sex-specific alternative splicing of RNA from the transformer gene results from sequence-dependent splice site blockage

Barbara A. Sosnowski, John M. Belote, Michael McKeown

Research output: Contribution to journalArticle

185 Scopus citations

Abstract

Sex-specific alternative splicing of RNA from the Drosophila transformer gene involves competition between two 3′ splice sites. In the absence of Sex-lethal activity (as in males), only one site functions; in the presence of Sex-lethal activity (as in females), both sites function. Information for sex-specific splice site choice is contained within the intron itself. Deletions of the splice site used in males lead to Sex-lethal-independent use of the otherwise female-specific site. The relative amounts of unspliced and spliced RNA derived from these mutant genes do not change with changes in Sex-lethal activity. Specific nucleotide changes in the non-sex-specific splice site do not affect splicing activity but eliminate Sex-lethal-induced regulation. A deletion removing material between the two splice sites does not eliminate sex-specific regulation, while a deletion of the female splice site leads to a female-specific increase in unspliced RNA. These results are consistent with a model in which female-specific factors block the function of the non-sex-specific 3′ splice site.

Original languageEnglish (US)
Pages (from-to)449-459
Number of pages11
JournalCell
Volume58
Issue number3
DOIs
StatePublished - Aug 11 1989

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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