Septin genes in channel catfish (Ictalurus punctatus) and their involvement in disease defense responses

Qiang Fu, Yun Li, Yujia Yang, Chao Li, Jun Yao, Qifan Zeng, Zhenkui Qin, Shikai Liu, Daoji Li, Zhanjian "John" Liu

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Septins are an evolutionarily conserved family of GTP-binding proteins. They are involved in diverse processes including cytokinesis, apoptosis, infection, neurodegeneration and neoplasia. In this study, through thorough data mining of existed channel catfish genomic resources, we identified a complete set of 15 septin genes. Septins were classified into four subgroups according to phylogenetic analysis. Extensive comparative genomic analysis, including domain and syntenic analysis, supported their annotation and orthologies. The expression patterns of septins in channel catfish were examined in healthy tissues and after infection with two major bacterial pathogens, Edwardsiella ictaluri and Flavobacterium columnare. In healthy channel catfish, most septin genes were ubiquitously expressed and presented diversity patterns in various tissues, especially mucosal tissues, proposing the significant roles septin genes may play in maintaining homeostasis and host immune response activities. After bacterial infections, most septin genes were regulated, but opposite direction in expression profiles were found with the two bacterial pathogens: the differentially expressed septin genes were down-regulated in the intestine after E. ictaluri infection while generally up-regulated in the gill after F. columnare infection, suggesting a pathogen-specific and tissue-specific pattern of regulation. Taken together, these results suggested that septin genes may play complex and important roles in the host immune responses to bacterial pathogens in channel catfish.

Original languageEnglish (US)
Pages (from-to)110-121
Number of pages12
JournalFish and Shellfish Immunology
Volume49
DOIs
StatePublished - Feb 1 2016
Externally publishedYes

Keywords

  • Catfish
  • Expression
  • Infection
  • Innate immunity
  • Septin

ASJC Scopus subject areas

  • Environmental Chemistry
  • Aquatic Science

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