Sensing nucleic acids with dimer nanoclusters

Cheng Chi, Mathew M Maye, Andrea L. Stadler, Daniel Van Der Lelie, Oleg Gang

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

DNA linked nanoparticles are applied as a sensor for single-stranded DNA detection. The detection is based on nanocluster dimer disassembly triggered by the binding of target nucleic acid strands. Target detection and disassembly kinetics are followed in real-time using dynamic light scattering. The observed disassembly process is in agreement with a two-step kinetic model. The DNA sensing is found to be selective down to the level of a single base mismatch, even in the presence of a high concentration of interference DNA. The method further provides label-free detection of DNA in minutes and demonstrates the use of this new class of nanoclusters as a powerful platform for nucleic acid detection.

Original languageEnglish (US)
Pages (from-to)1051-1057
Number of pages7
JournalAdvanced Functional Materials
Volume21
Issue number6
DOIs
StatePublished - Mar 22 2011

Fingerprint

Nanoclusters
Nucleic acids
nucleic acids
nanoclusters
Dimers
Nucleic Acids
DNA
dimers
deoxyribonucleic acid
Kinetics
Single-Stranded DNA
Dynamic light scattering
Target tracking
Labels
kinetics
Nanoparticles
strands
Sensors
light scattering
platforms

Keywords

  • detection
  • DNA
  • nanoclusters
  • nanoparticles

ASJC Scopus subject areas

  • Biomaterials
  • Electrochemistry
  • Condensed Matter Physics
  • Electronic, Optical and Magnetic Materials

Cite this

Chi, C., Maye, M. M., Stadler, A. L., Van Der Lelie, D., & Gang, O. (2011). Sensing nucleic acids with dimer nanoclusters. Advanced Functional Materials, 21(6), 1051-1057. https://doi.org/10.1002/adfm.201002152

Sensing nucleic acids with dimer nanoclusters. / Chi, Cheng; Maye, Mathew M; Stadler, Andrea L.; Van Der Lelie, Daniel; Gang, Oleg.

In: Advanced Functional Materials, Vol. 21, No. 6, 22.03.2011, p. 1051-1057.

Research output: Contribution to journalArticle

Chi, C, Maye, MM, Stadler, AL, Van Der Lelie, D & Gang, O 2011, 'Sensing nucleic acids with dimer nanoclusters', Advanced Functional Materials, vol. 21, no. 6, pp. 1051-1057. https://doi.org/10.1002/adfm.201002152
Chi, Cheng ; Maye, Mathew M ; Stadler, Andrea L. ; Van Der Lelie, Daniel ; Gang, Oleg. / Sensing nucleic acids with dimer nanoclusters. In: Advanced Functional Materials. 2011 ; Vol. 21, No. 6. pp. 1051-1057.
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