Abstract
DNA linked nanoparticles are applied as a sensor for single-stranded DNA detection. The detection is based on nanocluster dimer disassembly triggered by the binding of target nucleic acid strands. Target detection and disassembly kinetics are followed in real-time using dynamic light scattering. The observed disassembly process is in agreement with a two-step kinetic model. The DNA sensing is found to be selective down to the level of a single base mismatch, even in the presence of a high concentration of interference DNA. The method further provides label-free detection of DNA in minutes and demonstrates the use of this new class of nanoclusters as a powerful platform for nucleic acid detection.
Original language | English (US) |
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Pages (from-to) | 1051-1057 |
Number of pages | 7 |
Journal | Advanced Functional Materials |
Volume | 21 |
Issue number | 6 |
DOIs | |
State | Published - Mar 22 2011 |
Keywords
- DNA
- detection
- nanoclusters
- nanoparticles
ASJC Scopus subject areas
- General Chemistry
- General Materials Science
- Condensed Matter Physics