Selective incorporation of nitrile-based infrared probes into proteins via cysteine alkylation

Hyunil Jo; Robert M. Culik; Ivan V. Korendovych; William F. Degrado; Feng Gai

doi:10.1021/bi101711a

Selective incorporation of nitrile-based infrared probes into proteins via cysteine alkylation

Hyunil Jo, Robert M. Culik, Ivan V. Korendovych, William F. Degrado, Feng Gai

Research output: Contribution to journal › Article › peer-review

43 Scopus citations

Abstract

The nitrile stretching vibration is increasingly used as a sensitive infrared probe of local protein environments. However, site-specific incorporation of a nitrile moiety into proteins is difficult. Here we show that various aromatic nitriles can be easily incorporated into peptides and proteins via either thiol alkylation or arylation reaction.

Original language	English (US)
Pages (from-to)	10354-10356
Number of pages	3
Journal	Biochemistry
Volume	49
Issue number	49
DOIs	https://doi.org/10.1021/bi101711a
State	Published - Dec 14 2010
Externally published	Yes

ASJC Scopus subject areas

Biochemistry

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10.1021/bi101711a

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title = "Selective incorporation of nitrile-based infrared probes into proteins via cysteine alkylation",

abstract = "The nitrile stretching vibration is increasingly used as a sensitive infrared probe of local protein environments. However, site-specific incorporation of a nitrile moiety into proteins is difficult. Here we show that various aromatic nitriles can be easily incorporated into peptides and proteins via either thiol alkylation or arylation reaction.",

author = "Hyunil Jo and Culik, {Robert M.} and Korendovych, {Ivan V.} and Degrado, {William F.} and Feng Gai",

year = "2010",

month = dec,

day = "14",

doi = "10.1021/bi101711a",

language = "English (US)",

volume = "49",

pages = "10354--10356",

journal = "Biochemistry",

issn = "0006-2960",

publisher = "American Chemical Society",

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TY - JOUR

T1 - Selective incorporation of nitrile-based infrared probes into proteins via cysteine alkylation

AU - Jo, Hyunil

AU - Culik, Robert M.

AU - Korendovych, Ivan V.

AU - Degrado, William F.

AU - Gai, Feng

PY - 2010/12/14

Y1 - 2010/12/14

N2 - The nitrile stretching vibration is increasingly used as a sensitive infrared probe of local protein environments. However, site-specific incorporation of a nitrile moiety into proteins is difficult. Here we show that various aromatic nitriles can be easily incorporated into peptides and proteins via either thiol alkylation or arylation reaction.

AB - The nitrile stretching vibration is increasingly used as a sensitive infrared probe of local protein environments. However, site-specific incorporation of a nitrile moiety into proteins is difficult. Here we show that various aromatic nitriles can be easily incorporated into peptides and proteins via either thiol alkylation or arylation reaction.

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U2 - 10.1021/bi101711a

DO - 10.1021/bi101711a

M3 - Article

C2 - 21077670

AN - SCOPUS:78650156868

SN - 0006-2960

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SP - 10354

EP - 10356

JO - Biochemistry

JF - Biochemistry

IS - 49

ER -

Selective incorporation of nitrile-based infrared probes into proteins via cysteine alkylation

Abstract

ASJC Scopus subject areas

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