Selective incorporation of nitrile-based infrared probes into proteins via cysteine alkylation

Hyunil Jo, Robert M. Culik, Ivan V. Korendovych, William F. Degrado, Feng Gai

Research output: Contribution to journalArticle

32 Scopus citations

Abstract

The nitrile stretching vibration is increasingly used as a sensitive infrared probe of local protein environments. However, site-specific incorporation of a nitrile moiety into proteins is difficult. Here we show that various aromatic nitriles can be easily incorporated into peptides and proteins via either thiol alkylation or arylation reaction.

Original languageEnglish (US)
Pages (from-to)10354-10356
Number of pages3
JournalBiochemistry
Volume49
Issue number49
DOIs
StatePublished - Dec 14 2010
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry

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