Segmental isotopic labeling of ubiquitin chains to unravel monomer-specific molecular behavior

Carlos A. Castañeda, Liat Spasser, Sudhir N. Bavikar, Ashraf Brik, David Fushman

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

"Seeing" each monomer in ubiquitin chains: By combination of the chemical synthesis of one ubiquitin monomer with recombinant expression of its 15N-containing counterpart, the labeling of ubiquitins in non-enzymatically assembled di-ubiquitin chains was achieved, to enable monomer-specific studies in solution. This allowed the first characterization of the structure, dynamics, intrachain interactions, and ligand binding of Lys33-linked di-ubiquitin.

Original languageEnglish (US)
Pages (from-to)11210-11214
Number of pages5
JournalAngewandte Chemie - International Edition
Volume50
Issue number47
DOIs
StatePublished - Nov 18 2011

Keywords

  • chain structures
  • isotopic labeling
  • protein structures
  • semisynthesis
  • ubiquitin

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

Fingerprint Dive into the research topics of 'Segmental isotopic labeling of ubiquitin chains to unravel monomer-specific molecular behavior'. Together they form a unique fingerprint.

  • Cite this