Secretion of functional formate dehydrogenase in Pichia pastoris

Michelle Takacs, Olga V. Makhlynets, Patricia L. Tolbert, Ivan V. Korendovych

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Biofuels are an important tool for the reduction of carbon dioxide and other greenhouse emissions. NAD+-dependent formate dehydrogenase has been previously shown to be capable of the electrochemical reduction of carbon dioxide into formate, which can be ultimately converted to methanol. We established that a functional enzyme, tagged for immobilization, could be continuously secreted by Pichia pastoris. The protein can be easily separated from the growth media and its activity remains constant over an extended period of time. This is an important first step in creating a self-sustaining system capable of producing biofuels with minimal resources and space required.

Original languageEnglish (US)
Pages (from-to)381-386
Number of pages6
JournalProtein Engineering, Design and Selection
Volume30
Issue number5
DOIs
StatePublished - May 1 2017

Keywords

  • Formate dehydrogenase
  • Pichia pastoris
  • protein engineering

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Molecular Biology

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