SdeK is required for early fruiting body development in Myxococcus xanthus

Anthony Garza, Jeffrey S. Pollack, Baruch Z. Harris, Albert Lee, Ingrid M. Keseler, Ellen F. Licking, Mitchell Singer

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Abstract

Myxococcus xanthus cells carrying the Ω4408 Tn5lac insertion at the sde locus show defects in fruiting body development and sporulation. Our analysis of sde expression patterns showed that this locus is induced early in the developmental program (0 to 2 h) and that expression increases approximately fivefold after 12 h of development. Further studies showed that expression of sde is induced as growing cells enter stationary phase, suggesting that activation of the sde locus is not limited to the developmental process. Because the peak levels of sde expression in both an sde+ and an sde mutant background were similar, we conclude that the sde locus is not autoregulated. Characterization of the sde locus by DNA sequence analysis indicated that the Ω4408 insertion occurred within the sdeK gene. Primer extension analyses localized the 5' end of sde transcript to a guanine nucleotide 307 bp upstream of the proposed start for the SdeK coding sequence. The DNA sequence in the -12 and -24 regions upstream of the sde transcriptional start site shows similarity to the σ54 family of promoters. The results of complementation studies suggest that the defects in development find sporulation caused by the Ω4408 insertion are due to an inactivation of sdeK. The predicted amino acid sequence of SdeK was found to have similarity to the sequences of the histidine protein kinases of two-component regulatory systems. Based on our results, we propose that SdeK may be part of a signal transduction pathway required for the activation and propagation of the early developmental program.

Original languageEnglish (US)
Pages (from-to)4628-4637
Number of pages10
JournalJournal of Bacteriology
Volume180
Issue number17
StatePublished - Sep 1998
Externally publishedYes

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ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Garza, A., Pollack, J. S., Harris, B. Z., Lee, A., Keseler, I. M., Licking, E. F., & Singer, M. (1998). SdeK is required for early fruiting body development in Myxococcus xanthus. Journal of Bacteriology, 180(17), 4628-4637.